Joanna of Austria, Daughter of Charles V (1535–1573) 1554 Medalist: Jacopo Nizolla da Trezzo Italian Joanna of Austria, daughter of Charles V, married in 1553 Don Juan, son of the King Don Juan III of Portugal; he never ascended the throne, and after his death in 1554, she became the regent of Joanna of Austria, Daughter of Charles V (1535–1573) 195386

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