. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 262 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS ft. Raper, K. B. 1984. Pp. 19. 74-75 in The Dictyosteliads, Princeton University Press. Princeton. New Jerse> 7 Ruppel, K. M., T. Q. P. Uyeda, and J. A. Spudich. 1994. J. Biol. Cliein. 269: 18773-18780. X Kitayama, J. Dai. H. P. Ting-Beall. M. A. Titus, and T. Q. P. Uyeda. 1998. Mol. Biol. Cell 9: 387a. 9. Fukui, Y. 1976. Dev. Growth Differ. 18: 145-155. 10. Kavman, S. C., and M. Clarke. 1983. ./. Cell Biol. 97: 1001-11)10. Reference: Biol. Bull. 197: 262-263. (Octo
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 262 REPORTS FROM THE MBL GENERAL SCIENTIFIC MEETINGS ft. Raper, K. B. 1984. Pp. 19. 74-75 in The Dictyosteliads, Princeton University Press. Princeton. New Jerse> 7 Ruppel, K. M., T. Q. P. Uyeda, and J. A. Spudich. 1994. J. Biol. Cliein. 269: 18773-18780. X Kitayama, J. Dai. H. P. Ting-Beall. M. A. Titus, and T. Q. P. Uyeda. 1998. Mol. Biol. Cell 9: 387a. 9. Fukui, Y. 1976. Dev. Growth Differ. 18: 145-155. 10. Kavman, S. C., and M. Clarke. 1983. ./. Cell Biol. 97: 1001-11)10. Reference: Biol. Bull. 197: 262-263. (October 1999) Dynamic Confocal Imaging of Interphase and Mitotic Microtubules in the Fission Yeast, S. pombe P. T. Tran1, P. Maddox2, F. Chang1, and S. Inone (Marine Biological Laboratory, Woods Hole, Massachusetts 02543) In the fission yeast. S. pombe, microtubules are required for multiple cellular processes, including maintenance of cell polarity, positioning of cellular organelles. and mitosis. Thus, microtubules are dynamic polymers (1), remodeling themselves within the living cell throughout the cell cycle. Unfortunately, our current view of the cytoskeletal architecture of the fission yeast microtubule conies from immunofluorescence microscopy and electron microscopy of static, fixed cells (2). However, recent technical advances in wide-field epifluorescence imaging of microtubules, made possible by fusions of green fluo- rescent protein to tubulin (GFP-tubulin), have allowed direct ob- ' Columbia University. New York. New York 10032. 2 University of North Carolina. Chapel Hill, North Carolina 27514. servation of microtubule behavior in living fission yeast cells (3). We have now applied real-time confocal microscopy to GFP- tubulin in haploid fission yeast, and can report dynamic changes in the microtubule cytoskeleton with unprecedented spatial and tem- poral resolution. A wild type haploid strain of fission yeast was transformed with a plasmid carrying the GFP-a2/tubulin
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