. Biophysical science. Biophysics. 496 Absorption Spectrophotometry /26 : 5 little significance. The curves in Figure 11 show the type of results to be expected from a stopped-flow and an accelerated-flow reaction, both of which are exponential in their time course. More rapid reaction. Light Beam Photo Multiplier or Other Sensing Device Figure 10. Rapid flow apparatus. C1 and C2 are three-way stopcocks allowing one to fill the syringes from the storage tanks and then discharge the syringes into the mixing chamber M. The optical density changes are observed at d cm down the flow tube. rates ca
. Biophysical science. Biophysics. 496 Absorption Spectrophotometry /26 : 5 little significance. The curves in Figure 11 show the type of results to be expected from a stopped-flow and an accelerated-flow reaction, both of which are exponential in their time course. More rapid reaction. Light Beam Photo Multiplier or Other Sensing Device Figure 10. Rapid flow apparatus. C1 and C2 are three-way stopcocks allowing one to fill the syringes from the storage tanks and then discharge the syringes into the mixing chamber M. The optical density changes are observed at d cm down the flow tube. rates can be detected by flow-type measurements than by measurements in cuvettes. However, there are larger experimental errors associated with the flow-type measurements. 5. Split-Beam and Dual-Beam Spectrophotometers In absorption spectrophotometry, the light intensities transmitted by a standard and a test sample are compared. Errors will be introduced into the optical densities so measured if the output of the light source changes. Similarly, if one measures the transmitted intensity at several wavelengths first for the standard and then for the test sample, it is important to compare readings at exactly the same wavelength. These operations can be simplified and the errors reduced by using slightly more complex equipment, namely a split-beam spectrophotometer. In this, the beam from the monochromator is split so that it alternates rapidly, passing first through the standard and then the test sample. There- after, the split beam is recombined to fall on a common detector. The. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ackerman, Eugene, 1920-. Englewood Cliffs, N. J. , Prentice-Hall
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