. Biological structure and function; proceedings. Biochemistry; Cytology. INTEGRATED OXIDATIONS IN ISOLATED MITOCHONDRIA 73 observation the less obvious was the second slower rate. When io~^ to 10"* M-2 :4-dinitrophenol (DNP) was used to stimulate isocitrate oxidation an initial rapid rate was observed followed by a very much slower rate, which persisted for at least 15 min. When the phosphate concentration in the medium was reduced below 5 niM, linear rates of isocitrate oxidation occurred, both when ADP and DNIP. 0 12 3 4 5 Time (min) Fig. 3. The stimulation of isocitrate oxidation by m
. Biological structure and function; proceedings. Biochemistry; Cytology. INTEGRATED OXIDATIONS IN ISOLATED MITOCHONDRIA 73 observation the less obvious was the second slower rate. When io~^ to 10"* M-2 :4-dinitrophenol (DNP) was used to stimulate isocitrate oxidation an initial rapid rate was observed followed by a very much slower rate, which persisted for at least 15 min. When the phosphate concentration in the medium was reduced below 5 niM, linear rates of isocitrate oxidation occurred, both when ADP and DNIP. 0 12 3 4 5 Time (min) Fig. 3. The stimulation of isocitrate oxidation by malate. Three separate experiments are shown. In each case 10^ M DNP was added followed 4 min. later (*) either by i -o mM-L-malate or 10 niM -L,( + )—isocitrate, or isocitrate and then malate. DXP wert used to stimulate respiration. However, even in a medium containing a low concentration of phosphate, if the mitochondria were depleted partly of their endogenous substrates by preincubating them with DNP or ADP for 5 min., added isocitrate was not oxidized at ap- preciable rates for many minutes. The addition of low concentrations of malate, fumarate or higher concentrations of oxaloacetate led to a marked increase in oxygen consumption. In the absence of isocitrate, and under these conditions, malate, fumarate or oxaloacetate did not produce any marked oxygen uptake (Fig. 3).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
Size: 1349px × 1853px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookcollectionbiodiver, booksubjectbiochemistry
