The Biochemical journal, 1907 . rger quantity by a slightmodification of Vahlens method. 22 kilos, of ergot were extracted with water ; the extract was concentrated to 22litres, and precipitated witli 1500 grammes of barium hydrate; the precipitate waswashed with 13 litres of water. On removal of the excess of baryta from the filtrateand washings, by means of sulphuric acid (about 50 grammes of barium sulphate beingformed), the solution was concentrated i?i vacuo to a small bulk, mixed with 10 kilos,of silver sand, and dried completely in vacuo at 100°. The resulting product was extractedthree
The Biochemical journal, 1907 . rger quantity by a slightmodification of Vahlens method. 22 kilos, of ergot were extracted with water ; the extract was concentrated to 22litres, and precipitated witli 1500 grammes of barium hydrate; the precipitate waswashed with 13 litres of water. On removal of the excess of baryta from the filtrateand washings, by means of sulphuric acid (about 50 grammes of barium sulphate beingformed), the solution was concentrated i?i vacuo to a small bulk, mixed with 10 kilos,of silver sand, and dried completely in vacuo at 100°. The resulting product was extractedthree times with 92 per cent, alcohol, and the resulting alcoholic solution (about 30 litres) ERGOTOXINE AND CONSTITUENTS OF ERGOT 291 was evaporated. The dark brown residue was then dissolved in the minimum quantityof boiling 75 per cent, alcohol (3 litres) ; on standing a crystalline deposit was formed,which was recrystallised twice from 75 per cent, alcohol. The yield was 15 grammes,or about 0*07 per cent, of the ergot Fig. 8a Cat, 3 kilos. Early pregnancy. Urethane. Abdomen opened in saline bath at 37 . Record ofuterine contractions and carotid blood-pressure. Contraction of the uterus pulls down thelever, the movements being magnified of injecting 100 mgms. of clavin, dissolved in water, intravenously. No perceptiblealteration in uterine tone or rhythm. The arrow indicates the time of injection for thetracing from the uterus. For analysis the substance was recrystallised once with animalcharcoal, and finally once without animal charcoal. It melted in asealed capillary tube at 265°. Heated in an open tube, it yieldeda crystalline sublimate, but also underwent a slight decomposition,and gave an odour very similar to that which leucin gives whenheated in the same manner. The substance consisted of minutefeathery needles, but sometimes rhomb-shaped plates were copper acetate it yielded a blue copper salt, slightly soluble inwater. The gene
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