. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Ixodidae 255 REARING SMALL LOTS OF TICKS In some respects the rearing procedure and equipment described above, while adequate for the rearing of D. andersoni in large quantities, are not well adapted for the requirements of a small laboratory where a lesser number of ticks is required. Small numbers of ticks may be confined in cotton stoppered or muslin capped vials to permit ready observation. In the absence of thermal JF~l D D Fig. 57.—Section of tick feedin
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Ixodidae 255 REARING SMALL LOTS OF TICKS In some respects the rearing procedure and equipment described above, while adequate for the rearing of D. andersoni in large quantities, are not well adapted for the requirements of a small laboratory where a lesser number of ticks is required. Small numbers of ticks may be confined in cotton stoppered or muslin capped vials to permit ready observation. In the absence of thermal JF~l D D Fig. 57.—Section of tick feeding girdle for experimental purposes. A, threaded and flanged ring; B, cover; C, long adhesive band for girdle; D, short adhesive band covering toothed flange. cabinets ticks may be kept at room temperature in glass vials or card- board pill boxes almost in contact with moist sand. In cases where con- trolled humidity conditions are required the pill boxes or vials may be kept in ordinary desiccating jars containing solutions of salts that will provide the desired relative humidity. The tick feeding girdle shown in Figure 50, while simple and useful for routine tick feeding, is not adapted for experimental feeding of small groups of ticks where close observation and easy manipulation of the feeding ticks are desired. Once the adhesive tape is in place its partial removal in order to remove or replace ticks causes skin irritation and any active and un- attached ticks may escape. Therefore, a tin capsule made from the threaded end ring and cover used in cardboard mailing tubes is substituted for the brass gauze capsule used in mass feeding. A section diagram is shown in Figure 57. For complete details the reader is referred to Public Health Reports 1933, pp. Fig. 58.—Diagram of a cage designed for rearing ticks on small Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and
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