. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IN STONH CRAB LARVAE 45 Energy consumed was measured daily as the difference between the number of Artemia nauplii initially offered to each individual and those remaining after twenty-four hours. Since the concentration of Artemia has been shown to affect the rate of development in decapod larvae (Reeve, 1969a, 1969b; Mootz, 1973), each larva was provided with 100 Artemia nauplii in 20 ml of seawater. This was consistent with the food density present in cultures used to determine growth, molting frequency, calor
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IN STONH CRAB LARVAE 45 Energy consumed was measured daily as the difference between the number of Artemia nauplii initially offered to each individual and those remaining after twenty-four hours. Since the concentration of Artemia has been shown to affect the rate of development in decapod larvae (Reeve, 1969a, 1969b; Mootz, 1973), each larva was provided with 100 Artemia nauplii in 20 ml of seawater. This was consistent with the food density present in cultures used to determine growth, molting frequency, caloric content, and respiration. Replicate samples of larvae were taken every second day from hatching to day 24, counted, dried at room temperature in a desiccator, and weighed on an analytical balance to the nearest mg. The values were plotted on semi-logarithmic paper and a least squares linear regression line fitted to the data. Dry weight data were also obtained for animals that had just molted to the first juvenile crab stage and the exuvia for each stage. Standard deviations and confidence limits were cal- culated for these data. Caloric content was determined by wet oxidation in the presence of an acid- dichromate mixture (Maciolek, 1962; American Public Health Association ct a!., 1965) for larvae at two-day intervals, exuvia of each stage, first juvenile crabs, and newly-hatched Artemia nauplii. Standard deviations and confidence limits were calculated for the data. Feeding rates and growth expressed as dry weight were converted to energy units using the values obtained. Metabolic energy expenditure was measured on a daily basis through megalopa in an all-glass differential microrespirometer. The procedures were modified from Grunbaum, Siegel, Schnlx, and Kirk (1955). Readings were taken in the middle of the day to avoid any diurnal effects which were not considered in this study. The experimental animal was placed in the respiration chamber with ml filtered seawa
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
