. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MLC * N a Figure 1. Cytocentrifuged Botry/liis schlosseri hemocytes stained with May Griinwald-Giemsa solution, (a) Lymphocyte-like cell (LL) and hyaline amebocyte (HA); (b) macrophage-like cell (MLC; n: nucleus; v: vacuole); (cl nephrocyte (N) with several empty vacuoles (arrowheads). Bar = 10 /xm. cytes and pigment cells (6%-10% of circulating hemocytes) were not well preserved after cytocentrifugation; they ap- peared as giant cells with empty vacuoles (Fig. Ic). Response of unstimulated hemocytes to anti-cytokine antibod
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MLC * N a Figure 1. Cytocentrifuged Botry/liis schlosseri hemocytes stained with May Griinwald-Giemsa solution, (a) Lymphocyte-like cell (LL) and hyaline amebocyte (HA); (b) macrophage-like cell (MLC; n: nucleus; v: vacuole); (cl nephrocyte (N) with several empty vacuoles (arrowheads). Bar = 10 /xm. cytes and pigment cells (6%-10% of circulating hemocytes) were not well preserved after cytocentrifugation; they ap- peared as giant cells with empty vacuoles (Fig. Ic). Response of unstimulated hemocytes to anti-cytokine antibodies Using anti-IL-1-a and anti-TNF-a, only some phago- cytes and a few morula cells were labeled after immuno- peroxidase staining (Table 1). Thus, most HA, MLC, and morula cells were not immunoreactive with either antibody (Fig. 3). Moreover, no other cell-types stained positively for stimulated. <D Figure 2. Unstimulated (a, c) and stimulated (h. d) morula cells after immunoperoxidase staining with anti-cytokine antibodies, (a. b) Incubation with anti-IL-1-a antibody; (c. d) treatment with the TNF-a antibody. Bar = 15 jum. the two cytokines. No labeling was observed when non- immune sera were used. Response of stimulated hemocytes to anti-cytokine antibodies When monolayers of hemocytes were activated with ei- ther mannan or PMM, the number of immunoreactive morula cells and the intensity of their immunoreactivity were progressively augmented with increasing incubation times (Figs. 2, 4). The difference in the number of unstimu- lated and stimulated reactive morula cells was always sig- nificant (P cvtes to antibodies raised to human cytokines Antibodies'1 Cell type Anti-iL-1-a Anti-TNF-a Phagocytes'" Morula cells ± ± ± ± a Values are percentage of total hemocytes plus or minus the standard deviation. h Phagocytes include hyaline amoebocytes and macrophai -li;. Please note that these images are extracted from scanned page images that
Size: 2097px × 1192px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
