. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 182 J. E. HOSE ET 10 11 FIGURES I-4. Light micrographs of agranular, small granule hemocyte with deposits, small granule hemocyte without deposits, and large granule hemocyte, respectively, treated to show sites of prophenoloxi- dase. The first two cells have no reaction product. The granules (arrows) in the small granule hemocyte without deposits react as does the entire cytoplasm of the large granule hemocyte. All figures 2500X. FIGURES 5-8. Light micrographs of same cell types as in Figures I-4, treated with Sudan bl
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 182 J. E. HOSE ET 10 11 FIGURES I-4. Light micrographs of agranular, small granule hemocyte with deposits, small granule hemocyte without deposits, and large granule hemocyte, respectively, treated to show sites of prophenoloxi- dase. The first two cells have no reaction product. The granules (arrows) in the small granule hemocyte without deposits react as does the entire cytoplasm of the large granule hemocyte. All figures 2500X. FIGURES 5-8. Light micrographs of same cell types as in Figures I-4, treated with Sudan black B. Agranular and small granule hemocytes with deposits show dense reaction products in the cytoplasm which obscure the nucleus. The latter two cell types have minimal staining and the nucleus (N) is clearly observed. All figures 2500X. FIGURE 9. Transmission electron micrograph showing homogeneous and electron-dense granules (G) from a large granule hemocyte fixed with both glutaraldehyde and osmium and stained with uranyl acetate and lead citrate. 43,OOOX. FIGURE 10. Transmission electron micrograph showing a granule from a large granule hemocyte that was fixed only with glutaraldehyde. Thin sections were floated on a protease solution for 6 h and examined without stain. Note the low electron density and extraction of the granule core (C). 43,OOOX. FIGURE 11. Transmission electron micrograph of a granule from a large granule hemocyte prepared as in Figure 10 and then floated on a solution of a-amylase for 12 h. Note the low electron density of the granule and extraction of its core (C). 43,000x. a few acid phosphatase-positive granules as well. In contrast, small granule hemocytes lacking cytoplasmic deposits have from three to eight positive foci consistent with lysosomes. Half of these cells have only a few (0-3) positive granules while the re- maining small hemocytes contain over 30 positive granules. A few of the latter group, presumably transitional to large granule h
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
