. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 198 JAMES L. CONGLETON. 140 160 180 200 VOL(ml) WT62(gm) FIGTKE 5. Terminal concentration of excreted metabolic byproducts following asphyxiation of Typhlogobius in different volumes of seawater. Circles represent the calculated concentra- tion (arbitrary units) of a byproduct excreted at a constant rate throughout the period of asphyxiation. Triangles represent actual measured hydrogen ion concentrations. time and inversely proportional to the relative volume (Vol/Wt°-C2). An equation relating survival time to relative volu
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 198 JAMES L. CONGLETON. 140 160 180 200 VOL(ml) WT62(gm) FIGTKE 5. Terminal concentration of excreted metabolic byproducts following asphyxiation of Typhlogobius in different volumes of seawater. Circles represent the calculated concentra- tion (arbitrary units) of a byproduct excreted at a constant rate throughout the period of asphyxiation. Triangles represent actual measured hydrogen ion concentrations. time and inversely proportional to the relative volume (Vol/Wt°-C2). An equation relating survival time to relative volume has previously been derived, so the terminal byproduct concentration may be expressed: Cone. = k + (Vol/Wt0-62) Vol/'Wt0-82 where k is a constant. If this expression is plotted in arbitrary units against rela- tive volume (represented by the plotted curve in Figure 5) it may be seen that the estimated byproduct concentration begins to increase rapidly below a relative volume of about 25 to 35. Plotting of actual measured hydrogen ion concentra- tion (terminal pH) against relative volume in Figure 5 shows close agreement with this expression; hydrogen ion concentration is seen to increase rapidly below a relative volume of 25 to 35, corresponding to a pH of to Figures 3 and 4 indicate that survival time falls off rapidly below relative volumes of 25 to 35 and below a pH of to This suggests that the exponential decrease in asphyxial survival time of Typhlogobius with decreasing seawater volume may re- sult from accumulation of an excreted acid metabolite. Sampling of burrow habitat The area selected for sampling of Callianassa affinis burrow water and inter- stitial water was a shingle and sand beach at Point Loma, California. Most indi- viduals of Typhlogobius californiensis used in this study were collected in the imme- diate area. The sampling site was at an elevation of 25 to 30 cm above Mean Lower Low Water and remained exposed for hour
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