. Biological structure and function; proceedings. Biochemistry; Cytology. Fig. l/ml. Fe(CN)j"" 9. Ferricyanide assay of soluble, purified enzyme at different pH values. zlE/min. pH Fig. 10. Effect of pH on the activity of purified DPNH dehydrogenase. Solid line, at fixed (i -66 x io~^ m) ferricyanide concentration; dashed line, V^ax values. The pH values given are those of the reaction mixture at 30°. Buffers: 0-04 M phosphate (pH 5-5 to 8-5) or tris (above pH 8-5). moderate slope and the reaction kinetics are of zero order, while at pH 5-0 the slope is negligible (Fig. 9) and the re
. Biological structure and function; proceedings. Biochemistry; Cytology. Fig. l/ml. Fe(CN)j"" 9. Ferricyanide assay of soluble, purified enzyme at different pH values. zlE/min. pH Fig. 10. Effect of pH on the activity of purified DPNH dehydrogenase. Solid line, at fixed (i -66 x io~^ m) ferricyanide concentration; dashed line, V^ax values. The pH values given are those of the reaction mixture at 30°. Buffers: 0-04 M phosphate (pH 5-5 to 8-5) or tris (above pH 8-5). moderate slope and the reaction kinetics are of zero order, while at pH 5-0 the slope is negligible (Fig. 9) and the reaction assumes first order characteristics with respect to the substrate. As the pH is increased above 8-0, the slope approaches infinity and at pH 8-5 to 9 it intersects the abscissa and thus no satisfactory measure of F^ax can be obtained. At present no satisfactory explanation of this complex behaviour is Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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