. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 144 M. E. JONES, S. BLACK, R. M. FLYNN, F. LIPMANN VOL. 12 (1953) in the following manner: for each fiM of CoA (310 units = i fxM), 75 ixM of KBH4 in M KOH and 10 fxM of tris (hydroxymethyl) aminomethane buffer, pH 9, were added. The vessel was incubated for 15 minutes at 37°, at which time the contents were. //M mg/ml Fig. 2. Effect of magnesium con- centration of the rate of acetate- ATP-CoA reaction. Each tube con- tained, in I ml. of re
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 144 M. E. JONES, S. BLACK, R. M. FLYNN, F. LIPMANN VOL. 12 (1953) in the following manner: for each fiM of CoA (310 units = i fxM), 75 ixM of KBH4 in M KOH and 10 fxM of tris (hydroxymethyl) aminomethane buffer, pH 9, were added. The vessel was incubated for 15 minutes at 37°, at which time the contents were. //M mg/ml Fig. 2. Effect of magnesium con- centration of the rate of acetate- ATP-CoA reaction. Each tube con- tained, in I ml. of reaction mixture: 25 units COA; 10 /j,M ATP; 10 /nM potassium acetate; 200/<Mtris (hydroxymethyl) - aminomethane buffer, pH ; 50 /nM KF; 10 /nM glutathione; and aoo/iMNHjOH, neutralized to pH with KOH; ml (10 units) of yeast fraction 4 and MgClg as indicated. Final pH was The tubes were incu- bated at 37°, and ml. samples were taken at 13, 30, and 60 mi- nutes to determine the rate of the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Burk, Dean. Amsterdam, Houston, Elsevier Pub. Co.
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