. Carnegie Institution of Washington publication. For a full account of Japanese methods of making agar-agar consult a paper entitled " The Seaweed Industries of Japan," by Dr. Hugh M. Smith, in the Bulletin of the United States Bureau of Fisheries for 1904. In addition to beef bouillon, or in place of it, various sub- stances, organic and inorganic, may be added to the agar with advantage. The writer makes much use of litmus-lactose agar, which is made out of ordinary nutrient agar by adding i per cent milk-sugar and enough pure litmus water to give a pur- ple-red color. Glyccrin-ag
. Carnegie Institution of Washington publication. For a full account of Japanese methods of making agar-agar consult a paper entitled " The Seaweed Industries of Japan," by Dr. Hugh M. Smith, in the Bulletin of the United States Bureau of Fisheries for 1904. In addition to beef bouillon, or in place of it, various sub- stances, organic and inorganic, may be added to the agar with advantage. The writer makes much use of litmus-lactose agar, which is made out of ordinary nutrient agar by adding i per cent milk-sugar and enough pure litmus water to give a pur- ple-red color. Glyccrin-agar, mattose-agar, etc., may be made up with any amount of the sub- stance desired, generally i or 2 per cent. Formerly it was difficult to filter agar perfectly clear and it was therefore used less than gelatin, but in recent years it has been discovered that this difficulty may be overcome if the agar is first brought into complete solution by prolonged boiling or by a short boiling at a temperature somewhat above 100° C., , no°C. The writer formerly obtained filtered clear agar by soaking the snipped agar in 5 per cent acetic-acid water for some hours, after which a thin cloth was tied over the mouth of the beaker securely, and tap water allowed to run into it for an hour or more /. £•., until all trace of acid was removed. The softened agar was then put into the bouillon, boiled for two hours, and finally filtered through S. & S. filter. b Fig. 31.* *Fic. 31.—Red sea-weeds from which agar-agar is manufactured, a, Gelidium contemn Lam., one-third natural size; b, Gelidium subcostatum Lam., one-half natural size. Prom a colored Jap- anese chart showing " The principal aquatic plants of Japan," supposed to be an official publication. Original in the library of the United States Fish Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these
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