. Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954. Electrophysiology; Electrolytes; Electrolytes; Electrophysiology; Physiology, Comparative. 228 ELECTROLYTES IN BIOLOGICAL SYSTEMS represented by the passively moving chloride ions. The resistance of the shunt largely determines the value of the spontaneous skin potential. This must al- ways be lower than the E^IF of the sodium transporting mechanism. Experi- ments in which the voltage was maintained at different levels, for


. Electrolytes in biological systems, incorporating papers presented at a symposium at the Marine Biological Laboratory in Woods Hole, Massachusetts, on September 8, 1954. Electrophysiology; Electrolytes; Electrolytes; Electrophysiology; Physiology, Comparative. 228 ELECTROLYTES IN BIOLOGICAL SYSTEMS represented by the passively moving chloride ions. The resistance of the shunt largely determines the value of the spontaneous skin potential. This must al- ways be lower than the E^IF of the sodium transporting mechanism. Experi- ments in which the voltage was maintained at different levels, for a given piece of skin, showed a positive correlation between sodium outflux and skin poten- tials but a negative correlation for influx as well as net flux and potential. In- y = + r 5 P : < E _ o. Millivolt 20 40 60 80 100 120 Fig. 14. Dependence of net chloride (NaCl) transport across skin upon sivin potential. Calcium-free Ringer's solution on both sides of the skin. Rana pipiens (28). crease of sodium influx with increasing spontaneous skin potential was seen by Fuhrman and Ussing (8). Although this type of relationship is theoretically quite possible, it is somewhat puzzling, since under similar experimental con- ditions Koefoed-Johnsen, Levi and Ussing (39), as well as other workers (28, 46), have found decreasing Cl~ influx, outflux and net flux with increasing spon- taneous skin potential (fig. 14). The correlation between skin potential and active salt transport can also be deduced from two useful equations derived by Linderholm (45):. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Shanes, Abraham M. (Abraham Morris), 1917-; Society of General Physiologists. Washington, American Physiological Society


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