Report on the etiology and prevention of yellow fever . ssed intothe test tube by coupling one of theshort glass tubes with a hydrogen ap-paratus. The test tube is sustained inan inverted position, (see Fig. 3), so thatthe action of gravity may come intoplay in displacing the oxygen. Thisplan is more convenient than the pre-ceding one, and the only objection toit is the fact that a certain amount ofoxygen may be retained in the solidi-fied agar jelly covering the walls ofthe tube. But if the hydrogen ispassed for a considerable time it would certainly seem that the greaterpart of the oxygen mu
Report on the etiology and prevention of yellow fever . ssed intothe test tube by coupling one of theshort glass tubes with a hydrogen ap-paratus. The test tube is sustained inan inverted position, (see Fig. 3), so thatthe action of gravity may come intoplay in displacing the oxygen. Thisplan is more convenient than the pre-ceding one, and the only objection toit is the fact that a certain amount ofoxygen may be retained in the solidi-fied agar jelly covering the walls ofthe tube. But if the hydrogen ispassed for a considerable time it would certainly seem that the greaterpart of the oxygen must escape by diftusion from this thin any but the most strictly anaerobic organisms should growin a tube prepared in this way, if the medium is suitable for it. I have varied the medium at times by mixing the agar jelly with flesli-l)eptone-gelatin, or with blood serum. This is easily done when themethod last described is employed, but not when the hydrogen is allowedto bubble through the liquified medium; for in this case the addition. Fig. 3. ETIOLOGY AND PREVENTION OF YELLOW FEVEE. 107reportonetiology00ster
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