. Biological structure and function; proceedings. Biochemistry; Cytology. No DPN 0 2 4 6 8 10 l/m"L. Fe(CN)^"" Fig. 7. Competitive inhibition of soluble, purified dehydrogenase by 9 x 10" M DPN. Standard ferricyanide assay; DPXH concentration = i -5 x 10"* M DPNH X 10^ Fig. 8. Lineweaver-Burk plot of effect of DPNH concentration on activity. The values on the ordinate are maximal velocities of ferricyanide reduction (^max with respect to ferricvanide) corresponding to each concentration of DPNH at pH 74, 30 . tion beyond about 2 x lo-"* m fails to increase the
. Biological structure and function; proceedings. Biochemistry; Cytology. No DPN 0 2 4 6 8 10 l/m"L. Fe(CN)^"" Fig. 7. Competitive inhibition of soluble, purified dehydrogenase by 9 x 10" M DPN. Standard ferricyanide assay; DPXH concentration = i -5 x 10"* M DPNH X 10^ Fig. 8. Lineweaver-Burk plot of effect of DPNH concentration on activity. The values on the ordinate are maximal velocities of ferricyanide reduction (^max with respect to ferricvanide) corresponding to each concentration of DPNH at pH 74, 30 . tion beyond about 2 x lo-"* m fails to increase the measured rate, even under conditions where the inhibitory effect of excess substrate is elimina- ted, it is doubtful if this value represents a true "saturation" of the dehy- Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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