. Special pathology and therapeutics of the diseases of domestic animals. Veterinary medicine. Etiology, Pathogenicity. 741 cultures one end is frequently club-shaped, and sometimes branches (Fig. 125). Staining. The bacillus stains easily but not uniformly with aqueous as well as with carbolized aniline dyes, giving the appearance of seg- ments separated by clear spaces; the Gram and Gram-Weigert methods give negative results. Cultivation. The bacillus of abortion grows especially well in a pure oxygen at- mosphere, or in air with low oxygen con- tents, while in the total absence of oxygen it
. Special pathology and therapeutics of the diseases of domestic animals. Veterinary medicine. Etiology, Pathogenicity. 741 cultures one end is frequently club-shaped, and sometimes branches (Fig. 125). Staining. The bacillus stains easily but not uniformly with aqueous as well as with carbolized aniline dyes, giving the appearance of seg- ments separated by clear spaces; the Gram and Gram-Weigert methods give negative results. Cultivation. The bacillus of abortion grows especially well in a pure oxygen at- mosphere, or in air with low oxygen con- tents, while in the total absence of oxygen it grows only with difficulty, and in ordi- nary atmosphere only after it has gradually become accustomed to such environment. Cultures made according to these conditions give particularly good results in or upon gelatin-agar, a combination of agar, gelatin and blood serum, as well as in glucose-agar. Upon the surface of such media, kept at body temperature there appears very fine, Fig. 125. Cory neb acterium flat, conical, evenly rounded, on transmitted abortus infeotiosi. Two-day- light, bluish transparent colonies which even o^'^ ^^a.^ cultures; eartol f uchsin. if thinly seeded reach at most the size of a pin head. "When the material to be examined is mixed with the media there develop, at a fixed distance from the surface and downward, numerous similar colonies closely placed (Fig. 126). Coagulated calf serum becomes liquefied in the vicinity of the colonies. In peptone bouillon and in liquid blood serum, few small white granules form. Milk becomes coagulated. Potato cultures show a marked resemblance to a culture of the bacterium mallei (McFadyean & Stockman). Tenacity. In cultures the bacillus dies within two weeks (Nowak found, however, that agar cultures were even after two years still capable of development. A temperature of 55° C. will destroy the organism in 3 minutes. It is destroyed by corrosive sublimate solution in 15 seconds, by 1% carbolic acid i
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectveterin, bookyear1912
