. The microscope and microscopical methods. Microscopes; 1896. CH. VII.} COLLODION SECTIONING. 159 the chloroform is poured off and the carbol-xylene* clarifier (§ 302) added. In a few hours the imbedded mass will become as transparent as glass and the tissue will seem to have nothing around it. Sometimes the collodion remains white and opaque for a considerable time. So far as the writer has been able to judge, this is due to moisture. If one breathes on the mass too much while imbedding, or if it is very damp in the room, the opacity may result. Sometimes, in objects of consid- erable size,
. The microscope and microscopical methods. Microscopes; 1896. CH. VII.} COLLODION SECTIONING. 159 the chloroform is poured off and the carbol-xylene* clarifier (§ 302) added. In a few hours the imbedded mass will become as transparent as glass and the tissue will seem to have nothing around it. Sometimes the collodion remains white and opaque for a considerable time. So far as the writer has been able to judge, this is due to moisture. If one breathes on the mass too much while imbedding, or if it is very damp in the room, the opacity may result. Sometimes, in objects of consid- erable size, this may remain for a week. This is the exception, how- ever, and if the mass seems sufficiently hard and tough, the cutting may proc:ed even if the clarification is fcSfcSsiftfciVwSf. Fig. 132. Preparation Vials for Histology and Embryology. These represent the two vials, natural size, that have been found most useful. They are kept in blocks with holes of the proper size. In case the imbedding mass will not clarify after a few days the im- bedded object may be placed in 95% alcohol for a day for dehydration, and then passed through chloroform and into the clarifier. There is usually no trouble in getting the mass perfectly clear in this way. *The hydrocarbon xylene (C8HI0) is called xylol in German. In English, mem- bers of the hydrocarbon series have the termination "ene," while members of the alcohol series terminate in "; fThe imbedded object may remain in the castor xylene clarifier indefinitely without harm. The collodion grows somewhat tougher by a prolonged stay in it. After cutting all the sections desired at one time, the imbedded tissue is returned to the clarifier for future Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Gage, Simon Henry, 1851-19
Size: 886px × 2819px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1800, bookdecade1890, booksubjectmicroscopes, bookyear
