. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AERATED WATER DEOXYGENATED WATER FIGURE 1. Diagrammatic cut-away view of water bath showing components of flow- through respirometer: A, influent oxygen electrode; B, effluent oxygen electrode; C, bypass shunt; D, respiration chamber; E, magnetic stirrers; F, differential oxygen me'.er; G, integrat- ing chart recorder; H, peristaltic pump. A small bubble trap (not shown) was installed between components H and A. experiment anoxic sea water was siphoned into BOD (300-ml biochemical oxygen demand) bottles containing one animal
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. AERATED WATER DEOXYGENATED WATER FIGURE 1. Diagrammatic cut-away view of water bath showing components of flow- through respirometer: A, influent oxygen electrode; B, effluent oxygen electrode; C, bypass shunt; D, respiration chamber; E, magnetic stirrers; F, differential oxygen me'.er; G, integrat- ing chart recorder; H, peristaltic pump. A small bubble trap (not shown) was installed between components H and A. experiment anoxic sea water was siphoned into BOD (300-ml biochemical oxygen demand) bottles containing one animal each, and bottles were sealed until death occurred. Other animals in individual, perforated vials were placed as a group into 5 liters of anoxic sea water which was replaced daily ; whenever the 5-liter jar was opened for removal of dead animals, it was regassed with nitrogen. Control animals were maintained in continuously aerated sea water. Tolerance of aerial anoxia was determined by supporting animals on the rack of a desiccator over water and continuously gassing the water with nitrogen. Control animals in an aerial environment were likewise maintained, but underlying water was gassed with air. Aerial Vo2 was measured in a Gilson respirometer with 130-ml respirometry flasks and equivalent ballast. Filter paper wicks and 30% KOH were added to each flask side-arm to absorb COo. Each flask contained one animal and one milliliter of sea water to maintain water saturation of air. One hour was allowed for equilibration ; thereafter oxygen consumption was read at 30-min intervals. Oxygen consumption at decreasing oxygen tensions was measured by placing the animal into a ID (inner diameter) plastic tube with openings at both ends. The tube was wedged vertically against the wall of a BOD bottle, and a small stirring bar was placed at the center of the bottle. An oxygen electrode was fitted snugly into the bottle opening, and depletion of oxygen was recorded with a Beck- ma
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
