. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. endonucleases, those positive for D12S950, D12S1914, and D12S1987. Endonucleases Eco RI, Bam HI, Bgl II, and Eco RI with Bam HI were used to fragment the cosmids. A preliminary cosmid map. or contig, was developed for one of the markers tested (D12S1914). Endonuclease Digestion for Cosmids Positive for STS D12S1914. Cosmids positive for D12S1914 were cut with Eco RI, Bam HI, Eco RI and Bam HI together, and Bgl II (FIGURES 24 AND 25). The positive cosmids were clC6, c69B7, c88A9, C167H8, cl79Fl 1,
. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. endonucleases, those positive for D12S950, D12S1914, and D12S1987. Endonucleases Eco RI, Bam HI, Bgl II, and Eco RI with Bam HI were used to fragment the cosmids. A preliminary cosmid map. or contig, was developed for one of the markers tested (D12S1914). Endonuclease Digestion for Cosmids Positive for STS D12S1914. Cosmids positive for D12S1914 were cut with Eco RI, Bam HI, Eco RI and Bam HI together, and Bgl II (FIGURES 24 AND 25). The positive cosmids were clC6, c69B7, c88A9, C167H8, cl79Fl 1, cl89G12, cl92A8, and c200B8. Mapping migration distances of the cut cosmids in comparison with the migration of 1KB ladder DNA and bacteriophage lambda Hind III markers allowed a determination of the approximate sizes of the cosmid fragments (Figure 26). Using a combination of each digestion it was possible to produce a map of the cosmids relative to each other for the region of the STS rKiUR[:24. Eco RI endonuclease digestion of cosmids positive for STS marker DI2SI9I4 as electrophoresed on a ''/o agarose gel. Lanes 2 and 12 contain 1KB ladder. Lane 3 contains X Hind III DNA, Lane 4 has clC6, Lane 5 has c69B7, Lane 6 has c88A9, Lane 7 has c 167H8, Lane 8 has c 179F11, Lane 9 has c 189012. Lane 10 has c 192A8. and Lane 11 has C200B8. 40. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Belk, Stephanie Heather.
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Keywords: ., bookcentury1900, booksubjectchromo, booksubjectsweetbriarcollege
