. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 278 J. L. RAM /:/ .11. 150T 100 50 KCI contractions mean resting length = 248 ± 28 n=9 * , p<, paired t compared to pro —5HT ( — 2 min) -2024 Time post —5HT (min) Figure 3. Magnitude and time course of 5-HT potentiation of elicited contractions. Fibers were measured in images similar to those illustrated in Figure 2. High potassium (100 KCI ASW) pulses were given every two min. Fibers were exposed to 5-HT (10~6 M in ASW) for 1 min immediately prior to the 0 time point. (Sigma). When cells began appeari
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 278 J. L. RAM /:/ .11. 150T 100 50 KCI contractions mean resting length = 248 ± 28 n=9 * , p<, paired t compared to pro —5HT ( — 2 min) -2024 Time post —5HT (min) Figure 3. Magnitude and time course of 5-HT potentiation of elicited contractions. Fibers were measured in images similar to those illustrated in Figure 2. High potassium (100 KCI ASW) pulses were given every two min. Fibers were exposed to 5-HT (10~6 M in ASW) for 1 min immediately prior to the 0 time point. (Sigma). When cells began appearing in the medium, fibers in remaining muscle pieces were dispersed by gentle trit- uration. The resultant dissociated cells were washed by centrifuging and resuspending them in wash medium containing all ingredients of the dissociating medium ex- cept collagenase. Washed cells were plated onto glass cov- erslips in 30-mm plastic petri dishes and stored at 4°C in a humidified chamber. Cells were usually used within 1- 4 days, although viable, contractile cells have survived for as long as 10 days under these conditions. Experiments were done at room temperature (20-24°C) after allowing the cells to warm gradually for at least 30 min. A plexiglass insert having a central hole approximately 1 cm in diameter was clamped into the 30-mm petri dish. The insert formed a small chamber, approximately ml in volume. Medium was constantly pumped into the chamber ( ml/min) and removed by suction from a surface wick opposite the inflow. The dish was mounted on a movable stage of an inverted microscope. The shape and movement of muscle fibers were recorded by a VHS camcorder (RCA CC310). A videotape demonstrating many of the contractile and electrophysiological responses reported in this paper ("Dissociated Muscle Fibers of Aplysia," by J. L. Ram) is available from the authors upon request. Morphometric analysis was done by measuring still-images on the tape playback.
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
