. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 58 Y. K. MARUYAMA r 100 135 - a M V 40 -. 20 - - 20 NDJFMAMJJASONDJFMAMJJASONDJFMAMJJASOND 89 90 91 92 t Figure 3. Slide sampling at the study site. Abscissa, months from November 1989 through December 1992. Ordmate (left), number of placozoans obtained from slides at each sampling (squares, •); ordinate (right), percentage of placozoan-positive slides (circles. O). Slides (10-34, 2? on average) that had been placed at the study site for about 1 month (19-68 days, 35 days on average) were used for the observations. Filled ar
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 58 Y. K. MARUYAMA r 100 135 - a M V 40 -. 20 - - 20 NDJFMAMJJASONDJFMAMJJASONDJFMAMJJASOND 89 90 91 92 t Figure 3. Slide sampling at the study site. Abscissa, months from November 1989 through December 1992. Ordmate (left), number of placozoans obtained from slides at each sampling (squares, •); ordinate (right), percentage of placozoan-positive slides (circles. O). Slides (10-34, 2? on average) that had been placed at the study site for about 1 month (19-68 days, 35 days on average) were used for the observations. Filled arrows indicate the timing of large intermittent peaks (November 1990, September 1991, and August 1992). The peak in November 1989 was also probably in this class (open arrow). I M W 9 H I/I 0 (X appearing in October 1990 to January 1991. September 1991, and August 1992 (see Fig. 4). The placozoans obtained from substrate materials were, however, small. The animals collected from some samples ranged from 100 /xm to 400 /urn ( 1 32 ± 77 /am. mean ± SD of 52 individuals), and were frequently damaged at the periphery. Thus many of these placozoans had probably been fragmented during the sampling procedure. All, how- ever, exhibited a platelike morphology. Field sampling from 1993 throii^li 2/1/1(1 More recently. (August 1993-November 2000), sam- plings lor placo/.oans, on either slides or natural substrates. were continued at the study site at a frequency of 1-3 times per year. Placozoans were present in every year. Moreover, placozoans were also present at other sites in this area of Shirahama, including a suhtidal site and a tidal pool in Tanabe Bay as well as in Kanayama Bay. with the substrate or with the ambient seawater. Field sam- ples of stones in containers of ambient seawater were used for this purpose (n = 19). The stone from each sample was individually transferred to artificial seawater (ASW + stone), the combination was shaken, and the ASW and the retained ambient
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
