. Bulletin. Plant diseases -- United States. 42 to render the fluid yellowish, Ps. hyacinth i did not redden the fluid, but made it colorless, the bacterial precipitate becoming rosy or salmon- colored. Produces indol slowly in peptonized beef broth and in pep- tonized Uschinsky's solution; does not produce nitrites in these solutions. Does not reduce potassium nitrate to nitrite in peptonized beef bouillon. Not a strong-smelling germ. Not readily destroyed bj^ its own decomposition products except in media containing alcohol. Will not grow in the thermostat at 37° C, and grows verj- feebly on
. Bulletin. Plant diseases -- United States. 42 to render the fluid yellowish, Ps. hyacinth i did not redden the fluid, but made it colorless, the bacterial precipitate becoming rosy or salmon- colored. Produces indol slowly in peptonized beef broth and in pep- tonized Uschinsky's solution; does not produce nitrites in these solutions. Does not reduce potassium nitrate to nitrite in peptonized beef bouillon. Not a strong-smelling germ. Not readily destroyed bj^ its own decomposition products except in media containing alcohol. Will not grow in the thermostat at 37° C, and grows verj- feebly on some media and not at all on others at 34° to 35° C. Optimum tem- perature 28° to 30° C., or thereabouts. Minimum temperature approx- imately 4° C. Thermal death point (10 minutes' exposure) ° C.; nearly all the rods are killed at 47° and a great many at ° C. Did not grow at room temperatures after 6 days exjDOSure in alkaline beef broth in the thermo- stat at 35° to °. Does not grow well in Uschinsky's solution. Grows much better in Uschinsky's solution when j)eptone is added to it. Grows well with a bright yellow color on cylinders of steamed coconut flesh, standing with one end in distilled water. Pathogenic to hyacinths. Enters the plant through wounds, through the blossoms, etc., and multiplies in the vascular system, filling the ves- sels, especially those of the bulb, with a bright yellow slime consisting of bacteria. The walls of the vessels are destroyed and extensive cavi- ties are formed in the bundles. The parenchyma around the bundles is also involved, but only very slowlj-, the organism being a feeble de- stroyer of cell walls. The host plant is not rapidly destroyed, a year or more being neces- sary. The cells are first separated by solution of the middle lamella, but the wall itself seems to finally disappear. The cavities contain innu- merable bacteria mingled with fragments of the dissolved bundles and of the surrounding parenchyma.
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