. Pathological technique; a practical manual for workers in pathological histology and bacteriology. veral times, to insure intimate contact of the C02 with the alkali. Lastly,before removing the thumb, all the gas is allowed to collect in the closed branch * The reaction was noted by placing a drop of the fluid on delicate litmus-paper. The cultures were occasionally boiled to drive off any CO2. In nocase did the reaction with the litmus-paper change. PATHOGENIC BACTERIA AND FUNGI. 293 Variety a. One per cent, dextrose-bouillon (at 370 C). Total gas, approximately 1/2 ; H/C02 approximately 2/
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. veral times, to insure intimate contact of the C02 with the alkali. Lastly,before removing the thumb, all the gas is allowed to collect in the closed branch * The reaction was noted by placing a drop of the fluid on delicate litmus-paper. The cultures were occasionally boiled to drive off any CO2. In nocase did the reaction with the litmus-paper change. PATHOGENIC BACTERIA AND FUNGI. 293 Variety a. One per cent, dextrose-bouillon (at 370 C). Total gas, approximately 1/2 ; H/C02 approximately 2/1; reaction strongly per cent, lactose bouillon:As in dextrose-bouillon (with slight variation).One per cent, saccharose-bouillon : Gas-production slower than in the preceding, lasting fromseven to fourteen days. Total gas finally about 2/3;H/C02 nearly 3/2. The final reaction in the bulb maybe slightly acid or alkaline, according to the rate of /?. The same in all respects excepting as to its behavior insaccharose-bouillon. Neither gas nor acids are formed in Fig. 63.—Typhoid bacilli from a bouillon culture, showing characteristic irregu-larity in staining and variability in length; X 2000 (Wright and Brown). Bacillus of Typhoid Fever.—Synonyms: Bacillustyphi abdominalis; Bacillus typhosus; Typhoid bacillus. Morphology.—Medium-sized bacilli with rounded ends,generally short (Fig. 63), but sometimes long or thread-like, so that none may escape when the thumb is removed. If C02 was present, apartial vacuum in the closed branch causes the fluid to rise suddenly when thethumb is removed. After allowing the layer of foam to subside somewhat,the glass scale is again applied to the closed branch, and the amount of C02absorbed may thus be measured. In all cultures of this character thus far ex-amined the gas remaining was explosive in character, and probably explosive character of this residue is easily demonstrated as follows :
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