. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NARCOTICS AND PERMEABILITY 315 as far as we are aware the work of previous investigators in this field makes no such distinction, it can be expected to throw no real light on the question of the permeability of the erythrocyte to water. II As a first step in the separation of the two types of factors, it seemed important to obtain curves representing the entire course of hemolysis from its beginning until further change had ceased. This necessary type of information, which, as has been mentioned, has apparently not been supp
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NARCOTICS AND PERMEABILITY 315 as far as we are aware the work of previous investigators in this field makes no such distinction, it can be expected to throw no real light on the question of the permeability of the erythrocyte to water. II As a first step in the separation of the two types of factors, it seemed important to obtain curves representing the entire course of hemolysis from its beginning until further change had ceased. This necessary type of information, which, as has been mentioned, has apparently not been supplied by previous workers, is particularly easy to obtain by the method of one of the authors (Jacobs, 1930). A typical experiment is represented in Fig. 1 in which the erythrocytes were those of the ox and the narcotic was ethyl urethane. The blood in this, as in all the. 10 20 30 40 50 60 70 80 90 TIME-MINUTES FIG. 1. Course of hemolysis of ox blood in buffered M NaCl in the presence (lower curve) and absence (upper curve) of M ethyl urethane: pH, ; temperature, 20° C. other experiments here described, was thoroughly and almost instan- taneously mixed with the solutions used in the proportion of approxi- mately 1 to 500. Kymograph records were made of the ensuing hemolysis and from these the curves in the figure were reconstructed. Because of the enormous importance of pH and temperature changes in experiments involving equilibria, the precautions described by Jacobs and Parpart (1931) were employed in all such experiments, the pH being kept almost constant in this case at approximately , and the temperature at 20° C. An inspection of Fig. 1 shows very clearly that under the conditions of this experiment the effect of ethyl urethane upon the final end-point reached by the system is far more striking than any possible effect it might have upon the rate of hemolysis as such. Thus, in the absence of the urethane, a degree of hemolysis of approximately 61 per cen
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
