. Biological structure and function; proceedings. Biochemistry; Cytology. 400 500 700 800 900 600 X m ^ Fig. 5. Illustration of the correspondence between the absorption spectrum of the photosynthetic pigments in vivo and in the cell-free extracts. The divergence at lower wavelengths is attributable to differences in light scattering which were not completely compensated by the use of opal glass. Cells and debris are remo\ed from the crude extract by two successive centrifugations for 30 min. with refrigeration (5 ) at 26000 g. The "cleared" extract has an absorption spectrum which c
. Biological structure and function; proceedings. Biochemistry; Cytology. 400 500 700 800 900 600 X m ^ Fig. 5. Illustration of the correspondence between the absorption spectrum of the photosynthetic pigments in vivo and in the cell-free extracts. The divergence at lower wavelengths is attributable to differences in light scattering which were not completely compensated by the use of opal glass. Cells and debris are remo\ed from the crude extract by two successive centrifugations for 30 min. with refrigeration (5 ) at 26000 g. The "cleared" extract has an absorption spectrum which corresponds with the in vivo spectrum (Fig. 5). The small differences which are observed are attributable to differences in light scattering. This agreement provides some assurance that the phvsicochemical characteristics of the pigment bearer have not been disturbed greatly during the process of cell disintegration. A pigmented fraction, free from other macromolecular constituents, can be prepared by repetitive centrifugation for 2 hr. at 144 000 g under refrigeration {^ ). The progress of the fractionation is indicated by changes in the components observed in the analytical ultracentrifuge and by. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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