. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NEURODEVELOPMENT IN APLYS1A CAUFORNICA 309. Figure 2. Aplvsia californica during trochophore stages. Anterior is to the right in each figure. (A) Dorsal view of an embryo on day showing the two pairs of posteriorly located FMRFumide-LIR cells with anterior projections. Scale bar = 20 /mm. (B) Right lateral view of an embryo showing immunoreactivity for '^-tubulin on day 3. Displays the locations of the apical tuft, prototroch. telotroch, and anal cell in the trochophore. Scale bar = 25 /xm. (C) EFLRIamide-like immunoreac
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NEURODEVELOPMENT IN APLYS1A CAUFORNICA 309. Figure 2. Aplvsia californica during trochophore stages. Anterior is to the right in each figure. (A) Dorsal view of an embryo on day showing the two pairs of posteriorly located FMRFumide-LIR cells with anterior projections. Scale bar = 20 /mm. (B) Right lateral view of an embryo showing immunoreactivity for '^-tubulin on day 3. Displays the locations of the apical tuft, prototroch. telotroch, and anal cell in the trochophore. Scale bar = 25 /xm. (C) EFLRIamide-like immunoreactivity observed on day 3. showing F-rl, F-/1 and -12 (which are not in focus), and the plexus under the apical tuft (arrow). Scale bar = 25 (D) FMRFamide-like immunoreactivity observed in the same embryo as 2B showing F-rl, -r2, -1\ and -12, and plexus under the apical tuft. Scale bar = 25 /am. C and D demonstrate the similar pattern of immunoreactivity for EFLRlamide and FMRFamide. apical commissure toward the foot. Also by day 8, formal- dehyde glutaraldehyde-induced fluorescence, indicative of catecholamine-containing cells, was observed in the foot region. The catecholamine-containing foot cells were vase- shaped and appeared in two bilaterally symmetric groups of two to three cells on each side (see day 9, Figs. 4B, 6A, B). By day 9 additional FMRFamide-LIR cells were ob- served in positions consistent with the locations previously identified as the developing cerebral, pedal, and pleural ganglia of the future adult CNS in A. califoniicii (Krieg- stein, 1977; Marois and Carew, 1990; Marois and Carew, 1997b) and other opisthobranchs (Kempf et 1997a). One of these FMRFamide central cells (FC-1) appeared near F-rl, another (FC-2) was located near F-r2, and a third (FC-3) was observed to the left of the apical commissure (Figs. ID, 3C). Also by day 9, the number of catechol- amine-containing cells increased to four to five cells on each side of the foot (Figs. 4B,
Size: 1664px × 1501px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
