. Anatomischer Anzeiger. Anatomy, Comparative; Anatomy, Comparative. 458 Very similar results follow if strong solutions of salts are used. Thus in a supersaturated solution of KNO3 the sperms remain for hours folded up or with but incipient stages of unfolding. On the other hand sperms in very large amounts of water soon swell up into greatly distended spheres with the bowl projecting from one pole. That the sperms expand at a certain stage of dilution of the natural spermatic liquid and that strong salts retard or prevent this expansion seems to be due to a purely osmotic factor such as is s
. Anatomischer Anzeiger. Anatomy, Comparative; Anatomy, Comparative. 458 Very similar results follow if strong solutions of salts are used. Thus in a supersaturated solution of KNO3 the sperms remain for hours folded up or with but incipient stages of unfolding. On the other hand sperms in very large amounts of water soon swell up into greatly distended spheres with the bowl projecting from one pole. That the sperms expand at a certain stage of dilution of the natural spermatic liquid and that strong salts retard or prevent this expansion seems to be due to a purely osmotic factor such as is shown to controll the forms of some other decapod sperms by the work of Koltzoff. The initial stages in unfolding seen from the lower side as in Figure 2a, show radiating curved lines which upon focussing can be traced some distance into the interior. Those lines seem to be clefts, or vertical plates, in the sperm-substance below the vesicle. Whether ^ ij they are there before the sperm is acted upon by liquids was not determined, since the normal spermatic liquid is so refracting that details in the sperm are not readily made out. From a side Fig. 2. Beginnings of uncoiling of . ,, , , p a) Yiew of bottom, and b) view of vicw thosc early stagcs of ex- side of a sperm. pansiou give the appearance of faint, rings or spirally wound strands, Figure 2b, that look like highly refracting dots when seen in optical section at the edges of the sperm. The spiral lines are quite at the surface and external to the bowl about which they coil. The bottom of the bowl, which forms what we have called the top of the sperm, seems to be free from these coiled lines but the upper ends of these filaments are two fine to be traced. These spirals are the arms of the sperm shown in Figures 5 and 6. Each arm has an enlarged basal part that at first seems to be a ver- tical plate but later changes to become a more cylindrical base which is prolonged as the long terminal filament. The length of each arm i
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