. The Biological bulletin. Biology; Zoology; Marine biology. 176 KENNETH ALLEN AND J. AWAPARA Results 1) Formation of cystathionine Both M. edulis and R. cuneata were given a solution of S35-methionine (ap- proximately 500,000 counts). After 24 hours the organisms were extracted and the extracts fractionated. Fraction B in each case was chromatographed on paper. fi: ,- 4 - , ;, -• :%; •;:. : v: l Figure 1. Radioautograph of fraction B from M. ^u/u after the administration of S35-methionine. Solvents: phenol (P) and lutidine (L). Methionine (1), methionine sulfone . (2), hypotaurine (3) and cys
. The Biological bulletin. Biology; Zoology; Marine biology. 176 KENNETH ALLEN AND J. AWAPARA Results 1) Formation of cystathionine Both M. edulis and R. cuneata were given a solution of S35-methionine (ap- proximately 500,000 counts). After 24 hours the organisms were extracted and the extracts fractionated. Fraction B in each case was chromatographed on paper. fi: ,- 4 - , ;, -• :%; •;:. : v: l Figure 1. Radioautograph of fraction B from M. ^u/u after the administration of S35-methionine. Solvents: phenol (P) and lutidine (L). Methionine (1), methionine sulfone . (2), hypotaurine (3) and cystathionine (4). according to the method used by Simpson, Allen and Awapara (1959). using phenol-water in one direction and lutidine-water in the second direction. Radio- autographs of these chromatograms were made. The chromatograms from R. cuneata had no cystathionine. We cannot exclude its formation, for it could easily be a transient intermediate in this animal. It is very likely that it is formed, as we shall see in discussing formation of cystine. In Figure 1 is shown a radioautogram from M. edulis. Spot 4 is cystathionine and spot 3 is hypotaurine. Both of these. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-; Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report; HighWire Press. Lancaster, Pa. [etc. ] : Lancaster Press, inc. [etc. ]
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