. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HYDRA NEURONS DURING EMBRYOGENESIS 350 300 250 200 ISO 100 50 0 •J-S 1 D 20 d Cuticle • Minus? 0 Cigar B Tentacle Bud S5d B Adult B B n20d Cuticle • 0Cigar H Tentacle Bud H5d a Adult Ganglion Cells Sensory Cells Neuron Class Unclassifiable Neurons Figure 3. Mean (± SEM) numbers (a) and population densities (h) ot RFamide-positive ganglion cells, sensory cells, and unclassifiable neurons in different developmental stages of Hydra vulgaris (n = 5). One-way ANOVA tor numbers and population densities of ganglion cells across all
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HYDRA NEURONS DURING EMBRYOGENESIS 350 300 250 200 ISO 100 50 0 •J-S 1 D 20 d Cuticle • Minus? 0 Cigar B Tentacle Bud S5d B Adult B B n20d Cuticle • 0Cigar H Tentacle Bud H5d a Adult Ganglion Cells Sensory Cells Neuron Class Unclassifiable Neurons Figure 3. Mean (± SEM) numbers (a) and population densities (h) ot RFamide-positive ganglion cells, sensory cells, and unclassifiable neurons in different developmental stages of Hydra vulgaris (n = 5). One-way ANOVA tor numbers and population densities of ganglion cells across all six stages studied, sensory cells across the last four stages, and unclassi- tiable neurons across the last five stages showed significant differences (RFamide-positive ganglion cell number. F = 3 df = 5, P = ; density of RFamide-positive ganglion cell population. F = , dt = 5, P = ; RFamide-positive sensory cell number, F = ,dt = 3. P = ; density of RFamide-positive sensory cell population, F - , df = 3, P = ; RFamide-positive unclassifiable neuron number, F = df = 4, P = ; density of RFamide-positive unclassifi- ahle neuron population. F = df = 4, P = ). Results of post-hoc Tukey's honestly significant differences test are indicated with letters—bars sharing a letter within an ANOVA series are not significantly different (P > ). ing protocol. Though some double labeling was observed in each of the posthatching stages (« = 1 for double staining), the numbers of double-labeled neurons were minimal com- pared to the average total RFamide-positive and JD1-posi- tive neuron populations found in the single-stained samples (n = 5) of those stages. The percentage of double-labeled neurons ranged from a low of 1% of RFamide-positive neurons and 1% of JD1-positive neurons in the 5-d stage to a high of 5% of RFamide-positive neurons and 10% of JD1-positive neurons in the tentacle bud stage. Adult hydra sta
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
