Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . pirochaetae pallidae are suspected. (2). Preparation of Smears. To prepare blood smears, which are to be later examined in the stainedcondition, one may spread the blood in capillary layers on slides or between 444 DIAGNOSTIC METHODS. cover-glasses. The former method is the one used in the writers laboratoryand has given excellent and satisfactory results. A fair-sized drop of blood iscollected on one end of a clean dry slide, held between the thumb and secondand third finger of the l
Diagnostic methods, chemical, bacteriological and microscopical : a text-book for students and practitioners . pirochaetae pallidae are suspected. (2). Preparation of Smears. To prepare blood smears, which are to be later examined in the stainedcondition, one may spread the blood in capillary layers on slides or between 444 DIAGNOSTIC METHODS. cover-glasses. The former method is the one used in the writers laboratoryand has given excellent and satisfactory results. A fair-sized drop of blood iscollected on one end of a clean dry slide, held between the thumb and secondand third finger of the left hand. A second sHde is held in the same mannerby the right hand, but at an angle of 45 degrees to the first one and touchingthe drop of blood. Allow the blood to spread out by capillarity along of the second sHde. As soon as this occurs, draw the drop of bloodalong the first slide with a clean sweep, exerting little pressure with the secondslide and maintaining the angle of 45° between the two shdes, allowing thesecond slide to rest rather upon the blood than upon the slide (see cut). In the. Fig. 138—Preparation of smears with two glass slides. {Da Costa.) process, as recommended by some writers, the second slide is gradually drawninto a position perpendicular to the first one. This procedure does not yield,in the writers hands, as good results as the former method, as it is more difficultto maintain equal pressure, the smear being as a result too thick or too thinin places. Instead of a slide, a cigarette paper may be used as a spreaderand gives good results. This method of making blood smears has the advan-tage of offering a large surface for examination, of making smears which arefairly uniform after some practice, of dispensing with the necessity of mountingthe specimen, and of permitting the fixation of the smear in the free is less expensive than the method to be described later and permits of the
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectdiagnosis, bookyear19
