. Biomonitoring program : standard operating procedures. Water quality bioassay; Freshwater biology. INSTRUCTIONS FOR MOUNTING MIDGE LARVAE AND PAPAE HTSTRCrcnONS FOR I^UNITMG MIDGS LAR^/AE 1) Place larvae in a watch glass along with a sufficient volume of 70 alcohol to keep them covered; 2) Under low power on a dissecting scope, group the larvae according to size and general appearance. This usually results in midges of the same tribe or subfamily being mounted together, ^^*LLch in turn will aid in wor3<±ng specimens thrrjugh the keys more quickly; 3) Place a label on one end of a 25
. Biomonitoring program : standard operating procedures. Water quality bioassay; Freshwater biology. INSTRUCTIONS FOR MOUNTING MIDGE LARVAE AND PAPAE HTSTRCrcnONS FOR I^UNITMG MIDGS LAR^/AE 1) Place larvae in a watch glass along with a sufficient volume of 70 alcohol to keep them covered; 2) Under low power on a dissecting scope, group the larvae according to size and general appearance. This usually results in midges of the same tribe or subfamily being mounted together, ^^*LLch in turn will aid in wor3<±ng specimens thrrjugh the keys more quickly; 3) Place a label on one end of a 25 x 75 mm glass microscope slide. With the label to the ri^t, place three similar-sized larvae together on the slide, all with their heads pointed down; 4) Add enou<^ CMC-10 to surround the specimens. Spread out the mounting medium so it doesn't sit in a "bubble" around the specimens; 5) Excise the head capsules, making sure that each remains associated with its own boc^, and is positioned with the mouth parts (ventral side) up; 6) Place a glass cover slip (No. 1, 18 mm x 18 mm) over the specimens and, using forceps, apply gentle pressure to force o\it air bubbles and flatten the head capsules until the mouth parts spread out sli^tly; 7) Add additional mounting medium to edge necessary to rid the mount of air bubbles; the of the cover slip if 8) Record any useful observations about the specimens (, if ventral tubules are present, and how many pairs) on the SLIDE INVENTORY CATAIiOG SHEET. Refer to the specimens based on their position relative to the label: "A" indicating the cover slip closest to the label and "1" indicating the specimen closest to the label. Thus, Al is the specimen closest to the label and B3 is the furthest (see fig. 1).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble th
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Keywords: ., bookcentury1900, bookcontributorum, booksubjectfreshwaterbiology
