. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure I. With increasing time, the average length of F-actin decreases in the presence of polymerizing vimentin. Labeled, phalloidin-slabilized actin filaments (10 nM) are visible by fluorescence microscopy in a sample containing 18 fjM (1 mg/ml) unlabeled vimentin before and after addition of KCl to initiate vimentin polymerization. The dimension of each image is 50 fjm in width, and the image in panel 4 was taken 25 min after polymerization. Rheology of vimentin networks Rheology of vimentin networks carried out as d
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure I. With increasing time, the average length of F-actin decreases in the presence of polymerizing vimentin. Labeled, phalloidin-slabilized actin filaments (10 nM) are visible by fluorescence microscopy in a sample containing 18 fjM (1 mg/ml) unlabeled vimentin before and after addition of KCl to initiate vimentin polymerization. The dimension of each image is 50 fjm in width, and the image in panel 4 was taken 25 min after polymerization. Rheology of vimentin networks Rheology of vimentin networks carried out as de- scribed previously (Janniey etal., 1991). Oscillatory mea- surements of elastic modulus were made with a Rheome- trics RFS II fluids spectrometer (Rheometrics. Piscata- way, NJ) at a frequency of 1 rad/s and a strain amplitude of 1%. Results F-actin-vimentin interactions Labelled actin filaments were examined /;; vitro by fluorescence microscopy and were seen to break in the presence of polymerizing vimentin. Figure 1 shows a panel of four video frames: the fluorescent filaments are F-actin in a matrix of polymerizing unlabeled vimentin filaments. Such breakage was not seen under non-poly- merizing conditions, indicating that polymerization is re- quired. The decrease in average F-actin length is shown 18 |iM Polymerizing 7 \iM Polymerizing 7 nM Non-polymerizing. 10 15 20 25 30 Time [min] 40 Figure 2. Average length of F-actin decreases in the presence of polymerizing vimentin filaments. Higher concentrations of vimentin re- sult in faster rate of length decrease. Unpolymerized vimentin does not cause a decrease in F-aclin Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952
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