. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIVER-RNA-INDUCED FORMATION OF GUT 203 EE. FIGURE 3. A section through a tiny portion of a big skin vesicle, developed from a L-RNA-treated TPS. The attached mass of connective tissue (see also Fig. 7) is covered by a distinctive layer of endodermal epithelium, EE. < 100. FIGURE 4. A section through a poorly developed vesicle of the L-RNA-treated implants. V, intestinal vesicle and U, unidentified tissue. X 100. 10 anterior primitive streaks including Hensen's node were kept in the cold room for 15-18 hours. The explants
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIVER-RNA-INDUCED FORMATION OF GUT 203 EE. FIGURE 3. A section through a tiny portion of a big skin vesicle, developed from a L-RNA-treated TPS. The attached mass of connective tissue (see also Fig. 7) is covered by a distinctive layer of endodermal epithelium, EE. < 100. FIGURE 4. A section through a poorly developed vesicle of the L-RNA-treated implants. V, intestinal vesicle and U, unidentified tissue. X 100. 10 anterior primitive streaks including Hensen's node were kept in the cold room for 15-18 hours. The explants showed similar loose consistency as noted in the TPS. All developed into large or very large grafts containing skin, brain, intestine, cartilage, etc. Usually the implants were small but compact. When somewhat larger, they contained some connective tissue, blood (Fig. 1) and occasionally smooth muscle fibers. In cases when they were attached to the host, they were covered with poorly organized, vacuolated, spherical, or more or less elongated cells. These cells formed a definite layer in some cases and had the appearance of intestinal epithelium (arrow in Fig. 1). TABLE II Growth and differentiation of the posterior third primitive streak treated with and without liver protein () RNA and its hydrolytic products (A = total number, B = percentage of the total I? C = percentage of the recovered) Untreated 'control) Treated with Treated with RXA Treated with RXase- digested RXA Experimental series A B c A B C A B C A B C 1. Implants 60 — — . 33 — — 46 — — 29 — 2. Recovered 23 38 — • 13 39 — 27 59 — 23 79 — 3. Attached to host tissue 14 23 61 8 24 62 21 46 76 12 41 52 4. Size increased 3 or 6 10 26 2 6 15 16 35 59 14 48 61 more times 5. Differentiation 9 15 39 6 18 46 20 43 74 14 48 56 6. Epidermal 7 12 30 3 9 23 14 30 52 9 31 39 7. Epidermal with feather 3 5 13 2 6 15 9 20 33 9 31 39 buds 8. Endodermal 7 12 30 5 15 29 17 37 63 - 24 30 9. Gut 1 2 4 2
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
