Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . ch corresponds to the color of the blood stain theaccompanying haemoglobin value is read off* directly. Thisis a very convenient method for determining haemoglobin atthe bedside. There is a possibility of the colors being in-accurately printed, however, and even if originally correct intint, under the continued influence of air and light they musteventually alter somewhat. 5. Thoma-Zeiss Haemocytometer.—This is an instru-ment used in blood counting
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . ch corresponds to the color of the blood stain theaccompanying haemoglobin value is read off* directly. Thisis a very convenient method for determining haemoglobin atthe bedside. There is a possibility of the colors being in-accurately printed, however, and even if originally correct intint, under the continued influence of air and light they musteventually alter somewhat. 5. Thoma-Zeiss Haemocytometer.—This is an instru-ment used in blood counting, i. e., in determining the num-ber of erythrocytes and leucocytes. The instrument consists ). IM of a microscopic slide constructed of heavy glass and providedwith a central counting cell (see Fig. 70, below). This cell,with the cover glass in position, is exactly millimeter deep The floor of the cell is divided by delicate lines into squareseach of which i> 4(1)0- of a square millimeter in area (see Fig,7_, p. [83 ). The volume of blood therefore between any par-ticular square and the cover glass above must be ,,/,,,, cubic. Thoma-Z iixc; Chamber. (Da Costa.) millimeter. Accompanying each instrument are two capil-lary pipettes (Fig. 71, p. 182), each constructed with a mixingbulb in its upper portion. Each bulb is further provided withan enclosed glass bead which is of great assistance in mixingthe contents of the chamber. The stem of each pipette isgraduated in tenths from the tip to the bulb. The final grad-uation at the upper end of the bulb is 101 on the pipette usedin mixing the blood sample in which the erythrocytes arecounted (erythrocytometer, see Fig. 71, p. 182), and 11 on thepipette used in mixing the blood sample for the leucocytecount (leucocytometer,see Fig. 71. p. 182). In making bloodcounts with the haemocytometer it is necessary to use somediluting fluid. Two very satisfactory forms of fluid for thispurpose are Toisons and Sherringtons solutio
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