. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. RELATIONSHIP BETWEEN NUCLEAR VOLUMES, AMOUNT OF INTRANUCLEAR PROTEINS AND DESOXYRIBOSENUCLEIC ACID (DNA) IN VARIOUS RAT CELLS CECILIE LEUCHTENBERGKR AND FRANZ SCHRADER Institute of Pathology, Western Reserve University, Cleveland, Ohio, and Department of Zoology, Columbia University, Nciv York 27 The concept that increase in size of nuclei and cells within one tissue is directly correlated with an increase in chromosome number or chromosomal mass has become more or less accepted in cytology (Hertwig, 1934, 1939). But Montgom
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. RELATIONSHIP BETWEEN NUCLEAR VOLUMES, AMOUNT OF INTRANUCLEAR PROTEINS AND DESOXYRIBOSENUCLEIC ACID (DNA) IN VARIOUS RAT CELLS CECILIE LEUCHTENBERGKR AND FRANZ SCHRADER Institute of Pathology, Western Reserve University, Cleveland, Ohio, and Department of Zoology, Columbia University, Nciv York 27 The concept that increase in size of nuclei and cells within one tissue is directly correlated with an increase in chromosome number or chromosomal mass has become more or less accepted in cytology (Hertwig, 1934, 1939). But Montgomery (1910), Bowen (1922) and Schrader (1947) have showr that in different sizes of spermatocytes in pentatomid testes, the chromosome numbei and chromosome size are identical in the different sized cells. Recently, Schradei and Leuchtenberger (1950), using quantitative cytochemical methods, presented evidence that in the pentatomid Arvelius there exists a direct correlation between three sizes of nuclear volumes of spermatocytes (200, 400, 1600 cubic microns), and the amount of contained proteins, while the amount of DNA was the same in these nuclei. On the basis of these results, the assumption was made that changes in nuclear sizes in a tissue are due mainly to alterations in protein and water con- tent, and not due to different amounts of DNA. The present communication presents further evidence on a parallelism between size of nuclei and amount of intranuclear protein within one tissue, but points out that such a correlation does not apply to a tissue containing cells with different synthetic activities, such as the parietal and chief cells of the gastric mucosa. MATERIAL AND METHODS Rat tissue was fixed immediately after death in 50 per cent formalin and Carnoy and cut at different thicknesses, According to the requirements of the investigation. The estimation of DNA and protein was done by the cytochemical and photometric microscopic methods previously described (Schrader
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