. The Biological bulletin. Biology; Zoology; Marine biology. 218 AkDEAX 0 Cell ^Receptor site. ^-Anchor sites-. [Cell SYMMETRICAL FACTOR Receptor sites. -Anchor sites-. Factor adhesion sites ASYMMETRICAL FACTOR Figure 4. Symmetrical and asymmetrical factors. form a tight layer on the surface of aggregates of mechanically dissociated cells. Thus the CMF-dissociation procedure apparently alters the localization of cell types. Using a velocity sedimentation technique (Leith and Steinberg, 1972) it was possible to obtain a very distinct separation of the three cell types. The bottom fractio
. The Biological bulletin. Biology; Zoology; Marine biology. 218 AkDEAX 0 Cell ^Receptor site. ^-Anchor sites-. [Cell SYMMETRICAL FACTOR Receptor sites. -Anchor sites-. Factor adhesion sites ASYMMETRICAL FACTOR Figure 4. Symmetrical and asymmetrical factors. form a tight layer on the surface of aggregates of mechanically dissociated cells. Thus the CMF-dissociation procedure apparently alters the localization of cell types. Using a velocity sedimentation technique (Leith and Steinberg, 1972) it was possible to obtain a very distinct separation of the three cell types. The bottom fraction removed from the chamber consisted primarily of NBS+ cells. The central fraction contained the NBS" vacuolated cells and the upper fraction contained the NBS" collar cells. All three types of cells appeared healthy, and aggregated when factor was added. Cells from the NBS* fraction formed compact, rounded aggre- gates with little enclosed space. Vacuolated NBS" cells formed looser, irregularly shaped aggregates, while the NBS" collar cells aggregated poorly. When the NBS" cell fractions were combined with the NBS+ cells in the presence of factor, the aggre- gates consisted of clumps of NBS* cells surrounded by NBS" cells. Discussion The results reported here are consistent with the adhesive role for factor pro- posed by Humphreys, and they also give insight into a factor's mechanism of Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-; Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report; HighWire Press. Lancaster, Pa. [etc. ] : Lancaster Press, inc. [etc. ]
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