. Ciba Foundation symposium on drug resistance in micro-organisms: mechanisms of development. Drug resistance in microorganisms. Physiological Mechanisms of Resistance 169 residual enzyme activity (tested at 15°) after incubation of the extract at the temperatures and for the times indicated. A 60 MINUTES 120 Fig. 1. Effect of temperature on the stability of the pantothenate- synthesizing enzyme in extracts of mutant 99-1 tris-(hydroxy- methyl)-aminomethane (Tris) buffer pH 8 • 5 100; total volume 1 • 0 ml. After incubation for one hour samples were assayed for pantothenate as described i
. Ciba Foundation symposium on drug resistance in micro-organisms: mechanisms of development. Drug resistance in microorganisms. Physiological Mechanisms of Resistance 169 residual enzyme activity (tested at 15°) after incubation of the extract at the temperatures and for the times indicated. A 60 MINUTES 120 Fig. 1. Effect of temperature on the stability of the pantothenate- synthesizing enzyme in extracts of mutant 99-1 tris-(hydroxy- methyl)-aminomethane (Tris) buffer pH 8 • 5 100; total volume 1 • 0 ml. After incubation for one hour samples were assayed for pantothenate as described in the text. 100 per cent residual activity equals 86 m[jL moles of pantothenate per ml. per hour for 99-lt, 178 mjji. moles of pantothenate per ml. per hour for the wild type. O-Q- = mutant 99-lt; A-A- = wild type. (Reproduced by permission of University of Chicago Press.) large difference in thermal stability is seen. Furthermore, the fact that the difference resides in the enzymes themselves, rather than in their environments in the respective extracts,. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation; Wolstenholme, G. E. W. (Gordon Ethelbert Ward); O'Connor, Cecilia M. (Cecilia Mary), 1927-. Boston, Little, Brown
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