. Bonn zoological bulletin. Zoology. 82 Jouke R. Van der Zee & Rainer Sonnenberg. Fig. 10. SplitsTree network representation of the Aphyosemion dataset. Number of taxa = 26, 760 base pairs, fit = , uncor- rected p-distances, neighborNet. tween sequences are presented in Table 2, the observed values found between Aphyosemion and Mesoa- phyosemion as the outgroup range from , the maximum observed value within Aphyosemion is The samples of A. cognatum from aquarium strains orig- inating from Kinsuka and Lake Fwa are, despite nearly 900 km distance, identical, and also wit
. Bonn zoological bulletin. Zoology. 82 Jouke R. Van der Zee & Rainer Sonnenberg. Fig. 10. SplitsTree network representation of the Aphyosemion dataset. Number of taxa = 26, 760 base pairs, fit = , uncor- rected p-distances, neighborNet. tween sequences are presented in Table 2, the observed values found between Aphyosemion and Mesoa- phyosemion as the outgroup range from , the maximum observed value within Aphyosemion is The samples of A. cognatum from aquarium strains orig- inating from Kinsuka and Lake Fwa are, despite nearly 900 km distance, identical, and also with the shorter se- quence of an A. cognatum from Lake Fwa published by Murphy & Collier (1999, GenBank acc. no. AF002324), therefore it might be possible that the strains were erro- neously mixed or mislabelled since their introduction in the killifish hobby. A test with a second specimen from the Kinsuka strain resulted in the same sequence (data not shown). The A. cognatum 1515 sample is only represented by a female, so the species identification is tentative based on the knowledge of other Aphyosemion collections around this area and the resulting sequence is identical with a shorter sequence of Murphy & Collier (1999, GenBank acc. no. AF002327), which they have published as A. el- egans from Naoimda, and slightly different from their se- quence of what they call A. elegans from Madimba (Gen- Bank acc. no. AF002328). It is not clear if Naoimda is a misspelling of Madimba. However, the specimens from Madimba belong to A. cognatum ( see Seegers, 1997, p. 74), the sequences differ in only two bases. The phylogenetic analyses (Fig. 9) give an only partially resolved tree of the analysed specimens. In addition, there are differences in support for nodes; excluding the third protein coding positions gives no bootstrap support above 50% for several nodes, which were recovered by the oth- er analyses (Fig. 9). Aphyosemion musafirii seems to be closer to A. castaneum, howe
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