. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. Sequence-Tagged Site Marker D12S95U. Digoxigenin labeling of human DNA. K562, with primers 950F and 950R produced visible product at approximately 344 or 396 base pairs when electrophoresed. This demonstrated that the DNA for STS marker D12S950 was effectively labeled with digoxigenin. f he labeled sequence was then used as a probe for STS sequences within the cosmid dot blot during hybridization. Following film exposure eleven cosmids showed strong labeling reactions (FIGURE- 21). Those cosmids w
. The development of a cosmid map of chromosome 12p13. Sweet Briar College; Chromosomes. Sequence-Tagged Site Marker D12S95U. Digoxigenin labeling of human DNA. K562, with primers 950F and 950R produced visible product at approximately 344 or 396 base pairs when electrophoresed. This demonstrated that the DNA for STS marker D12S950 was effectively labeled with digoxigenin. f he labeled sequence was then used as a probe for STS sequences within the cosmid dot blot during hybridization. Following film exposure eleven cosmids showed strong labeling reactions (FIGURE- 21). Those cosmids with strong labeling were c64A5, c66G2. C133A5. cl64G4. cl67Fl. cl70G6, cl75FlK cl89C3. c203F5, c205G9, and c221 H6. These cosmids are most likely positive for the STS marker sequence. A positive control of cl70G6, already known to contain the STS marker through PCR, showed a strong labeling reaction on the blot, providing assurance that the system is accurate and 1 KiUKi 21. Dot Blot Hyhricli/ation of cosmid DNA with digoxigenin labeled D12S950 marker, the most distinct and darkest labeled dots were used to determine the identity of cosmids positive for the marker. These cosmids were c64A5, c66G2, cl33A5, cl64G4, cl67FI. CI70G6. CI75FI I. cl89C3. c203F5, c205G9, and c22IH6. Cosmid 179G6 was a positive control for the hybridization. 37. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Belk, Stephanie Heather.
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Keywords: ., bookcentury1900, booksubjectchromo, booksubjectsweetbriarcollege
