. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. BIOCHEMISTRY OF SABLEFISH 319 200 0) 100 Q. ' o o M "c. 40 50 60 70 FORKLENGTH (cm) 80 FIGURE 2. Pyruvate kinase (PK) activity (units per gram dry weight) versus forklength for Ano- plopoma fimbria collected off southern California. The equation generated by a linear regression is given, with the regression coefficient. variation with body size (multiple regression analysis). Thus, while differences among individuals in muscle water content contribute to the variation in enzymic activity (expressed per gram wet weight),
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. BIOCHEMISTRY OF SABLEFISH 319 200 0) 100 Q. ' o o M "c. 40 50 60 70 FORKLENGTH (cm) 80 FIGURE 2. Pyruvate kinase (PK) activity (units per gram dry weight) versus forklength for Ano- plopoma fimbria collected off southern California. The equation generated by a linear regression is given, with the regression coefficient. variation with body size (multiple regression analysis). Thus, while differences among individuals in muscle water content contribute to the variation in enzymic activity (expressed per gram wet weight), the only significant trend noted in this analysis is between length and enzymic activity expressed on a dry weight basis. The fact that significant correlations between enzymic activity on a wet weight basis were not found in the sablefish but were found in most other fishes studied may be due to the unusually large variation in water content found in the sablefish relative to other fishes so examined (cf. Siebenaller and Somero, 1982; Siebenaller el ai, 1982). For all comparisons of LDH and PK activities in different experimental groups we performed a correction for size-dependence of enzymic activities on the measured enzymic activities. For example, if a sablefish 66 cm in forklength was found to have a muscle LDH activity of 130 units activity per gram wet weight, and to have 82% water in its white muscle, the units of activity per gram dry weight would be 722 (130 X 100/18 =: 722). From the linear regression, the LDH activity increases by units per gram dry weight for each centimeter increase in length. All fish have been standardized by convention to a 55 cm "reference ; Thus, correcting the LDH activity of this 66 cm fish to 55 cm involves subtracting 6 units of LDH activity ( units/cm X 11 cm := 6 units), to yield a value of 716 units per gram dry weight. Converting back to units per gram wet weight, the size corrected LDH activity is 128. All LDH
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
