. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 220 D. O \ND D. J FFRMSSE-: HALF MOON BAY CAYUCOS. CYPRESS ?T-*2 MONTEREY figure I. Sampling sites for northeastern Pacific / <MI;. were as described by Ayala ct aL (1972) and for GPI by Tracey ct ul. (1975). The PEP-GL, PEP-PP and PEP- LVLT staining assays consisted of 30 ml of a 2'V agar solution (60°C) added to 20 mg of peptide substrate. 2000 units of peroxidase. 10 mg of O-Dianisidine. 5 mg of ('rt>ui/n\ iulainuiitcu\ toxin. ml of M Mn('l: and 20 ml ot' M Na:HPO4 butler. At least one McNeill Bay
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 220 D. O \ND D. J FFRMSSE-: HALF MOON BAY CAYUCOS. CYPRESS ?T-*2 MONTEREY figure I. Sampling sites for northeastern Pacific / <MI;. were as described by Ayala ct aL (1972) and for GPI by Tracey ct ul. (1975). The PEP-GL, PEP-PP and PEP- LVLT staining assays consisted of 30 ml of a 2'V agar solution (60°C) added to 20 mg of peptide substrate. 2000 units of peroxidase. 10 mg of O-Dianisidine. 5 mg of ('rt>ui/n\ iulainuiitcu\ toxin. ml of M Mn('l: and 20 ml ot' M Na:HPO4 butler. At least one McNeill Bay individual of known protein phenotype was included per gel to provide a standard electromorph. and specimens from all nine populations were repeatedly run together on the same gels to verify electromorph scoring. The right valves of 73 McNeill Bay specimens were retained and analy/ed for possible correlations of shell morphology and color to protein phenotype. Subsequent to the initial electrophoretic survey of Micii from the nine study sites (Fig. 1). a more detailed investigation of the McNeill Bay population was per- formed. This involved elcctrophoietic characterization of the proj.'1-nv off I) pair mating experiments. (2) speci- mens that lepi 'liiavd in isolation. (3) individuals that reproduced in the held. The degree of spatial overlap be- tween strains in tlu \1. \c-ill Bay study site was also elec- trophoretically assessed In February 1987. non-brooding ^acd adults were sampled from McNeill Bav and specimens were soiled according to shell color phenotype. Thirty pairs, each containing one white and one red shelled individual, were placed in separate culture vials containing 20 nils of 1 ^m tillered seawater. are positively thigmo- tactic (Morton. I960) and each pair was positioned in v lose physical contact to ensure mutual attachment with byssal threads to facilitate potential cross-fertilization. The culture vials were maintained at 18°Cand the cl
Size: 1555px × 1606px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
