. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ADENYLATE CYCLASE IN FW MUSSELS 601 400^ K^ 300 P i c HI 5 • o 100 0 ENDOGENOUS CALCIUM (™M/I) FIGURE 3. Gill adenylate cyclase activity varies inversely as a function of endogenous calcium in Lignmia subrostrata. Each point represents the mean value of three determinations. • = basal (n = 33), A = 10 ^Mj\ dopamine (n = 24), and • = 10 pM/l serotonin (n = 32) AC activities with the lines fit by linear regressions. dogenous Ca linear regression was calculated to have a slope of — 130 pmol cAMP (mg protein
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ADENYLATE CYCLASE IN FW MUSSELS 601 400^ K^ 300 P i c HI 5 • o 100 0 ENDOGENOUS CALCIUM (™M/I) FIGURE 3. Gill adenylate cyclase activity varies inversely as a function of endogenous calcium in Lignmia subrostrata. Each point represents the mean value of three determinations. • = basal (n = 33), A = 10 ^Mj\ dopamine (n = 24), and • = 10 pM/l serotonin (n = 32) AC activities with the lines fit by linear regressions. dogenous Ca linear regression was calculated to have a slope of — 130 pmol cAMP (mg protein • 5 min)~l/(mMCa/\) while slopes of both 5-HT and dopamine stimulated activities were higher (—763 ± 75 and —463 ± 19 [pmol cAMP (mg protein- 5 min)~1]/(mMCa/l), respectively). The effect of calcium on the monoamine stimulated activities was significantly different (P < ) from basal; the most pronounced effect was produced by 5-HT. Addition of exogenous calcium (CaCl2) inhibited purified pellet AC (Fig. 4). The effect of exogenous calcium was primarily on the monoamine stimulated AC activities. Monoamine stimulated AC activities were significantly inhibited (P < ). Normally, 5-HT and dopamine stimulated AC activities were and times above basal, respectively; in the presence of CaCl2, monoamine stimulated AC activity (above CaCl2 basal) was only and , respectively. Basal AC activity also was reduced by the addition of CaCl2. The addition of exogenous calcium (50% basal AC inhibition at a concentration mM/\) was not as effective as the endogenous calcium (about mM/1 calcium). The phosphodiesterase and non-specific phosphatase activities of the crude ho- mogenate pellet and the supernatant were studied to determine whether these enzyme. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
