. Directions for work in the histological laboratory, more especially arranged for the use of classes in the University of Michigan. Histology -- Laboratory manuals. — 145— paraffin to the toluol or xylol containing the tissue to be embedded; the dish or bottle containing said tissue can at this time be placed in the warm oven. In this way the change from the toluol or xylol to the melted soft paraffin can be made very gradual, and there will be less danger of causing any shrinkage in the tissue. (4) From the soft paraffin, the tissues are transferred to melted hard paraffin, which is usually
. Directions for work in the histological laboratory, more especially arranged for the use of classes in the University of Michigan. Histology -- Laboratory manuals. — 145— paraffin to the toluol or xylol containing the tissue to be embedded; the dish or bottle containing said tissue can at this time be placed in the warm oven. In this way the change from the toluol or xylol to the melted soft paraffin can be made very gradual, and there will be less danger of causing any shrinkage in the tissue. (4) From the soft paraffin, the tissues are transferred to melted hard paraffin, which is usually a mixture of equal parts of soft paraffin and a paraffin with a melting point of about 52^ to 55° C, and answers very well for ordinary room or laboratory temperature. During the summer months, it may be necessary to use two parts of the paraf- fin with 55° C. melting point, and only one of the soft, and in very warm weather even less of the soft. In ver}' warm weather the author has often used the following mixture with success : Soft paraffin ... 1 part by weight. Hard parnfiin ... 4 parts " " Spermaceti ... 1 part " " In the hard paraffin the tissues remain from 2 to 24 hours, of course in the warm oven. It is essential that the melted paraffins wliile in use for embedding should be at , c ^. "r Fig. I. Simple paraflBn bath; A, copper plate; B, paraffla tray; C, loop of filter paper; D, area of melted paraffla; E, area of uumelted paraffin; F, tripod; G, flame. a constant temperature; this can easily be done when a water-bath with temperature regulator, such as is found in most laboratories, is at hand. A very simple apparatus, and one that meets the requirements quite well, is shown. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Huber, Gotthelf Carl, 1865-. Arbor, Mich. G
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Keywords: ., bookcontributorgersteinuniversi, bookleafnumber157, bookyear1895
