. Bulletin of the Department of Agriculture. Agriculture. SACBKOOD. 29 In the study of the virus of sacbrood use has been made also of the Pasteur-Chamberland fiUer ' (fig. 27). This is a clay filter, the pores of which are much finer than those of the Berkefeld used. In using this filter, an aqueous suspension of larv^ dead of the disease is prepared as before. This is filtered by the aid of pressure obtained. ^' : ^T^'''''''* apparatus which can be employed m using the Pa5tem--Chamberland Berkefeld and other filters. Pasteur-Chamberlajid filter (b) with a glass mantle (a), a rubber


. Bulletin of the Department of Agriculture. Agriculture. SACBKOOD. 29 In the study of the virus of sacbrood use has been made also of the Pasteur-Chamberland fiUer ' (fig. 27). This is a clay filter, the pores of which are much finer than those of the Berkefeld used. In using this filter, an aqueous suspension of larv^ dead of the disease is prepared as before. This is filtered by the aid of pressure obtained. ^' : ^T^'''''''* apparatus which can be employed m using the Pa5tem--Chamberland Berkefeld and other filters. Pasteur-Chamberlajid filter (b) with a glass mantle (a), a rubber stopper (e) through which passes the filter, a connecting rubber tubing (d), glass tubmg (e), a perforated rubber stopper (f), a vacuum jar (g), designed by the writer, in which is placed a cotton-stoppered and steril- ized flask, a glass stopcock (h), a vacuum gauge (i), a reservoir (m) with pressure-rubber connections (J), and a vacuum pump (k). (Original.) by means of a partial vacuum in an apparatus devised for this pur- pose. Filtrates obtained from this filter when fed to healthy colonies produced the disease. Since the virus of sacbrood wiU pass through iThe Pasteur-Chamberland filter consists of clay molded in the form of a hollow cvlinder and baked 1 his IS used with a glass cylinder (a) fitted with a rubber stopper (c). In the use of this filter force is employed. This was obtained for these experiments through the use of a jar (g) devised by the writer in ^ hich a partial vacuum can be produced. Ln this jar, is placed a flask plugged with cotton'and sterilised ( onnecions are made as shown in the illustration, the vacuum bemg produced through the use of the pump (k). In less than half an hour usually a half-pint of filtrate can be obtained with this apparatus. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the origi


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Keywords: ., bookcentury1900, bookdecade1910, booksubjectagriculture, bookyear