. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 382 FINGERMAN, C. K. AXI) R. A. KRASNOW ml \(}Qr/c saline and assayed. This experiment was performed twice and the averaged results are presented in Figure 3. It is clear from inspection of this figure that once again the melanin-dispersing and white pigment-concentrating activities did not separate from each other. Although some chromatophorotropins from the fiddler crab have been analyzed previously by gel filtration (Fingerman and Couch, 1967; Fingerman and Rao, 1969a, 1969b), there has been no previous attem
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 382 FINGERMAN, C. K. AXI) R. A. KRASNOW ml \(}Qr/c saline and assayed. This experiment was performed twice and the averaged results are presented in Figure 3. It is clear from inspection of this figure that once again the melanin-dispersing and white pigment-concentrating activities did not separate from each other. Although some chromatophorotropins from the fiddler crab have been analyzed previously by gel filtration (Fingerman and Couch, 1967; Fingerman and Rao, 1969a, 1969b), there has been no previous attempt to assay the several fractions 20 15 a: - 10 c/) 0. 8 18 28 FRACTION NUMBER 38 FIGURE 4. The Standard Integrated Responses (SIR) of fiddler crabs evoked by the fractions obtained by passing an extract of fiddler crab eyestalks through a column of Bio-Gel P-6; dots, melanin-dispersing responses; circles, white pigment-dispersing responses; half-filled circles, red pigment-dispersing responses ; empty triangles, white pigment-concentrat- ing responses; half-filled triangles, red pigment-concentrating responses, arrow, void volume. simultaneously on the melanophores, leucophores and erythrophores. Conse- quently, definite conclusions concerning the elution patterns of the chromatophoro- tropins active on these three types of chromatophore were not possible. It was necessary to assay the fractions from the same chromatographic separation for several chromatophorotropic activities simultaneously if we were to attempt to determine the number of chromatophorotropins in the eyestalks of this fiddler crab. The resulting data could then be compared with those obtained (Fig. 2) after gel filtration of the extracts of the horseshoe crab CNS. The void volume of the column used to fractionate eyestalk extracts was 40 ml: the volume of each. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
