. Bonn zoological bulletin. Zoology. 268 Ulrich Joger & Ismail Bshaenia. Fig. 1. Tarentola mauritanica (Tunisia). Operational taxonomic units (OTUs) were defined using a combination of mitochondrial DNA clades and geo- graphic proximity. Linear Discriminant Function Analy- ses (LDFA) were used to find variables that separate the groups. Principal Component Analyses (PCA) were ap- plied to see whether groups are distinguishable without previous definition of OTUs. Significance of character dif- ferences were tested with t-tests. DNA was extracted from the preserved samples using standard pr
. Bonn zoological bulletin. Zoology. 268 Ulrich Joger & Ismail Bshaenia. Fig. 1. Tarentola mauritanica (Tunisia). Operational taxonomic units (OTUs) were defined using a combination of mitochondrial DNA clades and geo- graphic proximity. Linear Discriminant Function Analy- ses (LDFA) were used to find variables that separate the groups. Principal Component Analyses (PCA) were ap- plied to see whether groups are distinguishable without previous definition of OTUs. Significance of character dif- ferences were tested with t-tests. DNA was extracted from the preserved samples using standard procedures. Universal primers were used to am- plify mitochondrial 12S rRNA (372 bp) and 16s rRNA (2 fragments of448 and 604 bp). Sequences were determined using an automated sequencer, and aligned with CLUSTAL-W omitting gaps. Sequences from Genbank were added in some cases. A sequence of a paratype of the new subspecies was submitted to GenBank (IB47: Table 1. Distinguishing characters of North African Tarentola (mean ± standard deviation). Significant differences from T. sp. (Tunisia) are highlighted (in bold). Significance values are given for males (first value) and females separately (if different). *** ; ** P<; * P<; not significant. characters - Tunisia Tunisia Libya - N-Africa N=22 N=15 N=139 N=73 N=24 Maximal head 86 79 + body length Lamellae under ± ± ± *** ± *** ± *** 1st toe Lamellae under ± *** ± ± ± ** ± *** 4th toe Lamellae under ± ± ± ± ± *** 5th toe Ventral scales ± ± ± ± *** ± *** Dorsal tubercles ± ± ± ± **/*** ± Gular scales ± ± ± ± ± *** Interorbitals
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