. The chemistry and mode of action of plant growth substances; proceedings of a symposium held at Wye College, University of London, July 1955. Plant regulators; Auxin; Growth (Plants). Figure 25. Histogram showing the biological activity of a chromatogram run in hopropanol +ammonia-]-water (80; 10; 10) of an extract of 300 mg {fresh weight) of apical meristems of Adiatum pedatum, assayed with oat first internodes. Figure 24. Histograms showing the biological activity of chromatograms of tissue culture extracts run in hexane-\-water and assayed with first internode sections. PTN = extract with
. The chemistry and mode of action of plant growth substances; proceedings of a symposium held at Wye College, University of London, July 1955. Plant regulators; Auxin; Growth (Plants). Figure 25. Histogram showing the biological activity of a chromatogram run in hopropanol +ammonia-]-water (80; 10; 10) of an extract of 300 mg {fresh weight) of apical meristems of Adiatum pedatum, assayed with oat first internodes. Figure 24. Histograms showing the biological activity of chromatograms of tissue culture extracts run in hexane-\-water and assayed with first internode sections. PTN = extract with 2/3 ether+ 1/3 ethanol (3-5 hours at 0°C) of 4-5 g (fresh weight) of Parthenocissus tricuspidata normal tissue grown in vitro for 5 months on a medium containing sucrose, mineral salts, and lAA (50 yjL). PTH = extract with 2/3 ether + 1/3 ethanol (2 hours at 0°C) of A g (fresh weight) of Parthenocissus tricuspidata habituated' tissue, grown for 2 months on a medium containing ordy sucrose and mineral salts. PTG = extract with IjZ etker+lj'i ethanol (2 hours at O'C) of 3-75 g {fresh weight of Parthenocissus tricuspidata crown-gall tissue grown for three months on a medium containing only sucrose and mineral internodes. Figure 25 gives the results which demonstrate well that a good picture of the auxins present in plant tissues can be obtained with a crude extract, and with as little as 300 mg fresh weight of material. THE CHEMICAL IDENTIFICATION OF AUXINS ON THE CHROMATOGRAMS From the results presented so far, it is clear that one can detect numerous biologically active substances on the chromatograms of plant extracts. This, however, does not conclude our task. We want to know also the chemical 28. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Wye College; Wain, R. L; Wightman, F. (Frank). Londo
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Keywords: ., bookcentury1900, bookcollectionbiodiver, booksubjectgrowthplants
