Dental cosmos . ine stone and mounted inhard Canada balsam, which is warmed on a glass slide over a spirit-lampuntil fluid. 1278 THE DENTAL COSMOS. highest powers were employed, I found that the granular, appear-ance of the ground-substance was caused by the gelatin-yieldingdelicate fibrils being also stained somewhat black, while the calcifiedportions lying between them remained quite white. As the fibrilsrun nearly at right angles to the direction of the dentine-fibers, theyaccordingly appear as fine black dots in transverse sections (Figs. 14-16). In sections in which Tomess fibers are cut
Dental cosmos . ine stone and mounted inhard Canada balsam, which is warmed on a glass slide over a spirit-lampuntil fluid. 1278 THE DENTAL COSMOS. highest powers were employed, I found that the granular, appear-ance of the ground-substance was caused by the gelatin-yieldingdelicate fibrils being also stained somewhat black, while the calcifiedportions lying between them remained quite white. As the fibrilsrun nearly at right angles to the direction of the dentine-fibers, theyaccordingly appear as fine black dots in transverse sections (Figs. 14-16). In sections in which Tomess fibers are cut transversely, thefibrils can be traced in their course as delicate black lines. In Golgis method of staining, all organic constituents of thedentine are permeated by salts of silver and stained black, while thecalcified portions remain unchanged. The best way is to preparefairly thin ground sections of teeth preserved in absolute alcohol, totreat them after Golgis method, and finally to grind again both Fig. Fig. 15.—Reserve dentine from the root of a carious premolar. Wet groutid section, stainedwith Golgis method. 250 diam. surfaces upon a fine stone, and to polish them carefully. From thebeginning, only very thin sections should be used, because thickerones are not always completely permeated, and, in further grinding,unstained portions are met with. As by employing Golgis methodonly, all organic substance in the dentine is stained, some additionaltreatment is required to render the protoplasmic Tomess fiber dis-tinct from its Neumanns sheath. To effect this, I treated the sectionsfirst by v. Kochs petrifying method. By that means, from the pulpoutward, Tomess fibers, but not the dentine ground-substance, werepermeated with Canada balsam. By now staining the sections accord-ing to Golgi, the protoplasmic portions, permeated by balsam, re-mained unstained, and showed up distinctly from the black stainedground-substance. In transverse sections they will then have anappe
Size: 1584px × 1577px
Photo credit: © The Reading Room / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthor, bookcentury1800, bookdecade1890, booksubjectdentistry
