Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . ow 30° C. nor above 40° C; the optimum tempera-ture is 37° C, and the thermal death-point 56° C. It is an optional ana-erobe, non-motile, and does not pro-duce spores. In streak cultures onthe surface of serum a moist, shininggrowth develops along the line ofinoculation. The solidified serum isgradually liciuefied. forming a furrow,which at first extends in depth only,but later widens. Smear culturesdevelop as individual colonies, which])it the serum. After cultivationthrough a series
Manual of pathology : including bacteriology, the technic of postmortems, and methods of pathologic research . ow 30° C. nor above 40° C; the optimum tempera-ture is 37° C, and the thermal death-point 56° C. It is an optional ana-erobe, non-motile, and does not pro-duce spores. In streak cultures onthe surface of serum a moist, shininggrowth develops along the line ofinoculation. The solidified serum isgradually liciuefied. forming a furrow,which at first extends in depth only,but later widens. Smear culturesdevelop as individual colonies, which])it the serum. After cultivationthrough a series of serum tubes theorganism may, in some instances, begrown upon agar, where it then formstranslucent, discrete colonies, likedewdrops. In bouillon containingone-third serum a turbidity quicklydevelops, which is not lessened bythe occurrence of a grayish-white deposit on the sides and bottomof the tube. The organism requires frequent transplantation. Stf works referred tn in foot-note, page 81. For careful study of thisorganism sec paper by J. W. Eyre. M S >i fBact. vol. vi. .\<». I. May. Fig. 76.— —{H>insfU uml Surrt.) • o;o. p-itl. :>...! 134 GENERAL PATHOLOGY. Deuioiistratioii.—The Bacillus lacunatus stains slowly but clearlvwith the usual anilin dyes; the best results are obtained with an aqueoussolution of Bleu dc Ronx. The reaction to Grams stain is peculiar,partial decolorization taking place; the staining is never characteristic-ally that generally obtained by Grams method. This peculiaritv is tobe utilized in identifying the organism. Patliogaiesis.—The bacillus is not pathogenic for animals. In manit is found in cases of subacute and chronic conjunctivitis and may bepresent in large numbers. The disease has been produced experiment-ally by inoculating the human conjunctiva. The Bacillus pyocyaneus^ (Gessard, 18S2) is a short delicate rod i ,.to , long, ,. wide, motile, aerobic, and growing readily at temper
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